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验证数据展示

  • WB analysis using 28883-1-AP

    WB analysis using 28883-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28883-1-AP (CORO2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 28883-1-AP

    IHC staining of human skin cancer using 28883-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28883-1-AP (CORO2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of Jurkat using 28883-1-AP

    WB analysis of Jurkat using 28883-1-AP

    WB result of CORO2A antibody (28883-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CORO2A transfected Jurkat cells.

  • Featured Product
  • KD/KO验证

CORO2A Polyclonal antibody

CORO2A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28883-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, ELISA

别名

CLIPINB, CORO2A, Coronin 2A, DKFZp686G19226, IR10, WD repeat containing protein 2, WDR2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, Raji cells
Positive IHC detected inhuman skin cancer tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28883-1-AP targets CORO2A in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 CORO2A fusion protein Ag29696 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coronin, actin binding protein, 2A
别名 CLIPINB, CORO2A, Coronin 2A, DKFZp686G19226, IR10, WD repeat containing protein 2, WDR2
计算分子量 60 kDa
观测分子量48 kDa
GenBank蛋白编号BC000010
基因名称 CORO2A
Gene ID (NCBI) 7464
RRIDAB_2881227
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92828
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CORO2A (Coronin 2A), also named as CLIPINB, is a member of the 'short' coronin subfamily containing a single WD40-repeat domain. It has been shown that the expression of CORO2A is up-regulated in human colon cancer. CORO2A can interact with MAPK14 and PRMT5. The calculated MW of CORO2A is 60 kDa, while 28883-1-AP antibody detects 48 kDa protein which is similar to the paper published. (PMID: 26373535)

实验方案

Product Specific Protocols
WB protocol for CORO2A antibody 28883-1-APDownload protocol
IHC protocol for CORO2A antibody 28883-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols