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CORO2B Polyclonal antibody

CORO2B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13802-1-AP

Print datasheet

Synonyms

Protein FC96, KIAA0925, Coronin-like protein C, Coronin-2B, Coronin 2B

验证数据展示

  • WB analysis of Jurkat using 13802-1-AP

    WB analysis of Jurkat using 13802-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13802-1-AP (CORO2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 13802-1-AP

    IHC staining of human ovary cancer using 13802-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 13802-1-AP (CORO2B antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 13802-1-AP

    IHC staining of human ovary cancer using 13802-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 13802-1-AP (CORO2B antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, mouse brain tissue
Positive IHC detected inhuman ovary cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13802-1-AP targets CORO2B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CORO2B fusion protein Ag4793 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coronin, actin binding protein, 2B
Synonyms Protein FC96, KIAA0925, Coronin-like protein C, Coronin-2B, Coronin 2B
Calculated Molecular Weight 475 aa, 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC026335
Gene Symbol CORO2B
Gene ID (NCBI) 10391
RRIDAB_2087042
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UQ03
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CORO2B antibody 13802-1-APDownload protocol
IHC protocol for CORO2B antibody 13802-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols