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  • Featured Product
  • KD/KO Validated

COTL1 Monoclonal antibody, PBS Only

COTL1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4A3D9

Cat no : 60237-1-PBS

Print datasheet

Synonyms

CLP, Coactosin like protein, COTL1

验证数据展示

  • WB analysis of A431 using 60237-1-Ig (same clone as 60237-1-PBS)

    WB analysis of A431 using 60237-1-Ig (same clone as 60237-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 60237-1-Ig (COTL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 60237-1-Ig (same clone as 60237-1-PBS)

    IHC staining of human lung cancer using 60237-1-Ig (same clone as 60237-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60237-1-Ig (COTL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60237-1-Ig (same clone as 60237-1-PBS)

    IF Staining of HepG2 using 60237-1-Ig (same clone as 60237-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COTL1 antibody (60237-1-Ig, Clone: 4A3D9 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 60237-1-Ig (same clone as 60237-1-PBS)

    WB analysis of HeLa using 60237-1-Ig (same clone as 60237-1-PBS)

    WB result of COTL1 antibody (60237-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COTL1 transfected HeLa cells. This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

产品信息

60237-1-PBS targets COTL1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen COTL1 fusion protein Ag1230 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name coactosin-like 1 (Dictyostelium)
Synonyms CLP, Coactosin like protein, COTL1
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 14-16 kDa
GenBank Accession NumberBC010884
Gene Symbol COTL1
Gene ID (NCBI) 23406
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14019
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

COTL1, also named as CLP, binds to F-actin in a calcium-independent manner. And it has no direct effect on actin depolymerization.