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COX-1/Cyclooxygenase-1 Recombinant antibody

COX-1/Cyclooxygenase-1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241373C10

Cat no : 84151-4-RR

Print datasheet

Synonyms

PGH1, PTGS1, PGHS-1, EC:1.14.99.1, Cyclooxygenase-1

验证数据展示

  • WB analysis using 84151-4-RR

    WB analysis using 84151-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84151-4-RR (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of THP-1 using 84151-4-RR

    WB analysis of THP-1 using 84151-4-RR

    THP-1 cells were subjected to SDS PAGE followed by western blot with 84151-4-RR (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84151-4-RR

    Affinity and Kinetic Characterization of 84151-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84151-4-RR against Human COX-1/Cyclooxygenase-1 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inC2C12 cells, THP-1 cells, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84151-4-RR is COX-1/Cyclooxygenase-1 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen COX-1/Cyclooxygenase-1 fusion protein Ag4055 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)
Synonyms PGH1, PTGS1, PGHS-1, EC:1.14.99.1, Cyclooxygenase-1
Calculated Molecular Weight 599 aa, 69 kDa
Observed Molecular Weight 60-72 kDa
GenBank Accession NumberBC029840
Gene Symbol PTGS1
Gene ID (NCBI) 5742
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP23219
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTGS1(Prostaglandin G/H synthase 1) belongs to the prostaglandin G/H synthase family and catalyzes the conversion of arachidonic acid to prostaglandin H2, which is subsequently metabolized to various biologically active prostaglandins. It is also named as COX1(Cyclooxygenase-1). There is no PTGS1 expression in fetal samples (prostate, seminal vesicles, or ejaculatory ducts), and only minimal expression in adult tissues (PMID: 10999846). PTGS1 has some isoforms with MW of 56-72 kD and PTGS1 can form homodimer.

实验方案

Product Specific Protocols
WB protocol for COX-1/Cyclooxygenase-1 antibody 84151-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols