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COX11 Polyclonal antibody

COX11 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11498-1-AP

Print datasheet

Synonyms

COX11, COX11P

验证数据展示

  • WB analysis of mouse liver using 11498-1-AP

    WB analysis of mouse liver using 11498-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11498-1-AP (COX11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 11498-1-AP

    IHC staining of human pancreas cancer using 11498-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11498-1-AP (COX11 antibody) at dilution of 1:50 (under 10x lens).

  • IP experiment of mouse liver using 11498-1-AP

    IP experiment of mouse liver using 11498-1-AP

    IP result of anti-COX11 (IP:11498-1-AP, 4ug; Detection:11498-1-AP 1:1000) with mouse liver tissue lysate 4400ug.

经过测试的应用

Positive WB detected inmouse liver tissue, mouse brain tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11498-1-AP targets COX11 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen COX11 fusion protein Ag2033 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name COX11 homolog, cytochrome c oxidase assembly protein (yeast)
Synonyms COX11, COX11P
Calculated Molecular Weight 276 aa, 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC005895
Gene Symbol COX11
Gene ID (NCBI) 1353
RRIDAB_10598173
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6N1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for COX11 antibody 11498-1-APDownload protocol
IHC protocol for COX11 antibody 11498-1-APDownload protocol
IP protocol for COX11 antibody 11498-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

J Neurosci

Golgi-Dependent Copper Homeostasis Sustains Synaptic Development and Mitochondrial Content.

Authors - Cortnie Hartwig
View Article
humanWB

J Mol Biol

COA6 Facilitates Cytochrome c Oxidase Biogenesis as Thiol-reductase for Copper Metallochaperones in Mitochondria.

Authors - David Pacheu-Grau
View Article
humanIHC

Sci Rep

Comprehensive analysis of copper-metabolism-related genes about prognosis and immune microenvironment in osteosarcoma

Authors - Zili Lin
View Article
humanWB

Neurosci Lett

RANBP2 mutation causing autosomal dominant acute necrotizing encephalopathy attenuates its interaction with COX11.

Authors - Akiko Shibata
View Article