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COX18 Polyclonal antibody

COX18 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18071-1-AP

Print datasheet

Synonyms

OXA1L2, Cytochrome c oxidase assembly protein COX18, mitochondrial, COX18Hs

验证数据展示

  • IF Staining of HepG2 using 18071-1-AP

    IF Staining of HepG2 using 18071-1-AP

    Immunofluorescent analysis of HepG2 cells, using COX18 antibody 18071-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • WB analysis of mouse liver using 18071-1-AP

    WB analysis of mouse liver using 18071-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 18071-1-AP (COX18 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse liver tissue, HeLa cells, NIH/3T3 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:300-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

18071-1-AP targets COX18 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen COX18 fusion protein Ag12709 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name COX18 cytochrome c oxidase assembly homolog (S. cerevisiae)
Synonyms OXA1L2, Cytochrome c oxidase assembly protein COX18, mitochondrial, COX18Hs
Calculated Molecular Weight 333 aa, 37 kDa
Observed Molecular Weight 37-40 kDa, 28-30 kDa
GenBank Accession NumberBC101684
Gene Symbol COX18
Gene ID (NCBI) 285521
RRIDAB_10638443
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N8Q8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for COX18 antibody 18071-1-APDownload protocol
IF protocol for COX18 antibody 18071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta

The mitochondrial TMEM177 associates with COX20 during COX2 biogenesis.

Authors - Isotta Lorenzi
View Article