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  • Featured Product
  • KD/KO Validated

COX2/ Cyclooxygenase 2/ PTGS2 Monoclonal antibody, PBS Only

COX2/ Cyclooxygenase 2/ PTGS2 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3G2B9

Cat no : 66351-1-PBS

Print datasheet

Synonyms

COX2, PTGS2, 3G2B9, EC:1.14.99.1, Cyclooxygenase-2

验证数据展示

  • WB analysis using 66351-1-Ig (same clone as 66351-1-PBS)

    WB analysis using 66351-1-Ig (same clone as 66351-1-PBS)

    Non-treated and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66351-1-Ig (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66351-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 66351-1-Ig (same clone as 66351-1-PBS)

    IHC staining of human cervical cancer using 66351-1-Ig (same clone as 66351-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66351-1-Ig (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66351-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COX2/PTGS2 antibody included
  • IHC staining of human colon cancer using 66351-1-Ig (same clone as 66351-1-PBS)

    IHC staining of human colon cancer using 66351-1-Ig (same clone as 66351-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66351-1-Ig (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66351-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COX2/PTGS2 antibody included
  • IF Staining of mouse lung using 66351-1-Ig (same clone as 66351-1-PBS)

    IF Staining of mouse lung using 66351-1-Ig (same clone as 66351-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using COX2/ Cyclooxygenase 2/ PTGS2 antibody (66351-1-Ig, Clone: 3G2B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66351-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy COX2/PTGS2 Ready-To-Use IHC Kit. COX2/PTGS2 primary antibody included.

产品信息

66351-1-PBS targets COX2/ Cyclooxygenase 2/ PTGS2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen COX2/ Cyclooxygenase 2/ PTGS2 fusion protein Ag24721 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
Synonyms COX2, PTGS2, 3G2B9, EC:1.14.99.1, Cyclooxygenase-2
Calculated Molecular Weight 604 aa, 68 kDa
Observed Molecular Weight 66-70 kDa
GenBank Accession NumberBC013734
Gene Symbol COX2/PTGS2
Gene ID (NCBI) 5743
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35354
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

COX2 (Prostaglandin G/H synthase 2, PTGS2) mediates the formation of prostaglandins from arachidonate. Its subunit structure is homodimer. The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa (PMID:19656660). It also expresses a band of 39 kDa after unspecific cleavage (PMID:17509125). The 50 kDa band of fragmented PTGS2 has also previously been detected in AD brains (PMID:14724276).