COX6B1 Polyclonal antibody
COX6B1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, Caco-2 cells, HeLa cells, HepG2 cells |
| Positive IHC detected in | human liver cancer tissue, human gliomas tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
产品信息
11425-1-AP targets COX6B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | COX6B1 fusion protein Ag1984 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cytochrome c oxidase subunit Vib polypeptide 1 (ubiquitous) |
| Synonyms | COX6B, COX VIb-1, COX VIb 1 |
| Calculated Molecular Weight | 80 aa, 10 kDa |
| Observed Molecular Weight | 10-13 kDa |
| GenBank Accession Number | BC001015 |
| Gene Symbol | COX6B1 |
| Gene ID (NCBI) | 1340 |
| RRID | AB_2085449 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14854 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COX6B1 (Cytochrome c oxidase subunit 6B1) is also named as COX6B and belongs to the cytochrome c oxidase subunit 6B family. It connects the two COX monomers into the physiological dimeric form. Defects in COX6B1 are a cause of mitochondrial complex IV deficiency (MT-C4D).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for COX6B1 antibody 11425-1-AP | Download protocol |
| IHC protocol for COX6B1 antibody 11425-1-AP | Download protocol |
| IF protocol for COX6B1 antibody 11425-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Biol PDGFRβ translocates to the nucleus and regulates chromatin remodeling via TATA element-modifying factor 1. | ||
Hum Reprod Posttranslational lysine 2-hydroxyisobutyrylation of human sperm tail proteins affects motility. | ||
J Biol Chem The cellular stress proteins CHCHD10 and MNRR1 (CHCHD2): Partners in mitochondrial and nuclear function and dysfunction. | ||
Mol Med Rep Overexpression of COX6B1 protects against I/R‑induced neuronal injury in rat hippocampal neurons. |
