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验证数据展示

  • WB analysis using 11437-1-AP

    WB analysis using 11437-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11437-1-AP (COX6B2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 11437-1-AP

    IHC staining of mouse testis using 11437-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11437-1-AP (COX6B2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 11437-1-AP

    IHC staining of mouse testis using 11437-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11437-1-AP (COX6B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

COX6B2 Polyclonal antibody

COX6B2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11437-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

COX VIb-2, Cytochrome c oxidase subunit 6B2, Cytochrome c oxidase subunit VIb, testis-specific isoform

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

11437-1-AP targets COX6B2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 COX6B2 fusion protein Ag1983 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytochrome c oxidase subunit VIb polypeptide 2 (testis)
别名 COX VIb-2, Cytochrome c oxidase subunit 6B2, Cytochrome c oxidase subunit VIb, testis-specific isoform
计算分子量 88 aa, 11 kDa
观测分子量 11 kDa
GenBank蛋白编号BC064548
基因名称 COX6B2
Gene ID (NCBI) 125965
RRIDAB_10859092
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6YFQ2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COX6B2(Cytochrome c oxidase subunit 6B2) encodes the testis-specific cytochrome C oxidase subunit VIb. It has been shown to be expressed in a subset of lung and breast tumors at a level >1% of the testicular level of expression(PMID: 24491090). It belongs to the cytochrome c oxidase subunit 6B family.

实验方案

Product Specific Protocols
WB protocol for COX6B2 antibody 11437-1-APDownload protocol
IHC protocol for COX6B2 antibody 11437-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

PMID: 20870728

Authors - Oliva Claudia R CR
View Article
mouseWB

Cell Death Dis

TMEM232 is required for the formation of sperm flagellum and male fertility in mice

Authors - Xinying Cai
View Article