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COX6B2 Polyclonal antibody

COX6B2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11437-1-AP

Print datasheet

Synonyms

Cancer/testis antigen 59, COX VIb 2, COX6B2, COXVIB2, CT59

验证数据展示

  • WB analysis using 11437-1-AP

    WB analysis using 11437-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11437-1-AP (COX6B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 11437-1-AP

    IHC staining of mouse testis using 11437-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11437-1-AP (COX6B2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 11437-1-AP

    IHC staining of mouse testis using 11437-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11437-1-AP (COX6B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11437-1-AP targets COX6B2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen COX6B2 fusion protein Ag1983 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome c oxidase subunit VIb polypeptide 2 (testis)
Synonyms Cancer/testis antigen 59, COX VIb 2, COX6B2, COXVIB2, CT59
Calculated Molecular Weight 88 aa, 11 kDa
Observed Molecular Weight 11 kDa
GenBank Accession NumberBC064548
Gene Symbol COX6B2
Gene ID (NCBI) 125965
RRIDAB_10859092
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6YFQ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COX6B2(Cytochrome c oxidase subunit 6B2) encodes the testis-specific cytochrome C oxidase subunit VIb. It has been shown to be expressed in a subset of lung and breast tumors at a level >1% of the testicular level of expression(PMID: 24491090). It belongs to the cytochrome c oxidase subunit 6B family.

实验方案

Product Specific Protocols
WB protocol for COX6B2 antibody 11437-1-APDownload protocol
IHC protocol for COX6B2 antibody 11437-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

PMID: 20870728

Authors - Oliva Claudia R CR
View Article