COX8A Polyclonal antibody
COX8A Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, human skeletal muscle tissue |
| Positive IHC detected in | human heart tissue, human hepatocirrhosis tissue, rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy COX8A Ready-To-Use IHC Kit. COX8A primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
15368-1-AP targets COX8A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | COX8A fusion protein Ag2885 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cytochrome c oxidase subunit 8A (ubiquitous) |
| Synonyms | COX, COX8, COX8 2, COX8A, COX8L, VIII, VIII L |
| Calculated Molecular Weight | 8 kDa |
| Observed Molecular Weight | 8 kDa |
| GenBank Accession Number | BC063025 |
| Gene Symbol | COX8A |
| Gene ID (NCBI) | 1351 |
| RRID | AB_10697832 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P10176 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COX8A(Cytochrome c oxidase subunit 8A) is also named as COX8, COX8L and belongs to the cytochrome c oxidase VIII family. This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. The gene encodes a full length protein of 8 kDa with a transit peptide of 25 amino acid.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for COX8A antibody 15368-1-AP | Download protocol |
| IHC protocol for COX8A antibody 15368-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis O-GlcNAcylation on Rab3A attenuates its effects on mitochondrial oxidative phosphorylation and metastasis in hepatocellular carcinoma. | ||
J Proteomics MALDI-MS tissue imaging identification of biliverdin reductase B overexpression in prostate cancer. | ||
Nat Commun A FRET-based respirasome assembly screen identifies spleen tyrosine kinase as a target to improve muscle mitochondrial respiration and exercise performance in mice | ||
Basic Res Cardiol Comparison of the stage-dependent mitochondrial changes in response to pressure overload between the diseased right and left ventricle in the rat |
