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Carboxypeptidase A3 Polyclonal antibody

Carboxypeptidase A3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16236-1-AP

Print datasheet

Synonyms

CPA3, MC-CPA, MC CPA, Mast cell carboxypeptidase A, EC:3.4.17.1

验证数据展示

  • WB analysis of mouse pancreas using 16236-1-AP

    WB analysis of mouse pancreas using 16236-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16236-1-AP (Carboxypeptidase A3 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 16236-1-AP

    IHC staining of human tonsillitis using 16236-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human small intestine using 16236-1-AP

    IHC staining of human small intestine using 16236-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody at dilution of 1:50 (under 40x lens).

  • IHC staining of human tonsillitis using 16236-1-AP

    IHC staining of human tonsillitis using 16236-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse pancreas tissue
Positive IHC detected inhuman tonsillitis tissue, human pancreas tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16236-1-AP targets Carboxypeptidase A3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen Carboxypeptidase A3 fusion protein Ag8984 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name carboxypeptidase A3 (mast cell)
Synonyms CPA3, MC-CPA, MC CPA, Mast cell carboxypeptidase A, EC:3.4.17.1
Calculated Molecular Weight 417 aa, 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC012613
Gene Symbol Carboxypeptidase A3
Gene ID (NCBI) 1359
RRIDAB_2085856
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15088
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Carboxypeptidase A3 antibody 16236-1-APDownload protocol
IHC protocol for Carboxypeptidase A3 antibody 16236-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Am J Pathol

Rare Pulmonary Connective Tissue Type Mast Cells Regulate Lung Endothelial Cell Angiogenesis.

Authors - Yue Ren
View Article