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Carboxypeptidase A5 Polyclonal antibody

Carboxypeptidase A5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13731-1-AP

Print datasheet

Synonyms

EC:3.4.17.-, CPA5

验证数据展示

  • WB analysis of PC-3 using 13731-1-AP

    WB analysis of PC-3 using 13731-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 13731-1-AP

    IHC staining of human testis using 13731-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human testis using 13731-1-AP

    IHC staining of human testis using 13731-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of PC-3 using 13731-1-AP

    IF Staining of PC-3 using 13731-1-AP

    Immunofluorescent analysis of PC-3 cells using 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inPC-3 cells
Positive IHC detected inhuman testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13731-1-AP targets Carboxypeptidase A5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Carboxypeptidase A5 fusion protein Ag4755 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name carboxypeptidase A5
Synonyms EC:3.4.17.-, CPA5
Calculated Molecular Weight 436 aa, 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC039362
Gene Symbol CPA5
Gene ID (NCBI) 93979
RRIDAB_2261098
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WXQ8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Carboxypeptidase A5 antibody 13731-1-APDownload protocol
IHC protocol for Carboxypeptidase A5 antibody 13731-1-APDownload protocol
IF protocol for Carboxypeptidase A5 antibody 13731-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols