验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue |
| Positive IHC detected in | human colon cancer tissue, human liver tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CPB2 Ready-To-Use IHC Kit. CPB2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
10672-1-AP targets Carboxypeptidase B2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse, rat |
| 免疫原 | Carboxypeptidase B2 fusion protein Ag1110 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | carboxypeptidase B2 (plasma) |
| 别名 | Carboxypeptidase B2, carboxypeptidase B2 (plasma), Carboxypeptidase U, CPB2, CPU, PCPB, Plasma carboxypeptidase B, TAFI |
| 计算分子量 | 48 kDa |
| 观测分子量 | 53 kDa |
| GenBank蛋白编号 | BC007057 |
| 基因名称 | Carboxypeptidase B2 |
| Gene ID (NCBI) | 1361 |
| RRID | AB_2086000 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96IY4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CPB2(Carboxypeptidase B2) belongs to the peptidase M14 family. The CPB2 gene encoding TAFI is a candidate gene for blood pressure. Circulating CPB2 and platelets may potentially contribute to regulating the bioactivity of leukocyte chemoattractant chemerin (RARRES2), and further extend the molecular link between blood coagulation/fibrinolysis and CMKLR1-mediated immune responses. This protein has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Carboxypeptidase B2 antibody 10672-1-AP | Download protocol |
| IHC protocol for Carboxypeptidase B2 antibody 10672-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
FEMS Immunol Med Microbiol Secreted Bacillus anthracis proteases target the host fibrinolytic system. | ||
Neurochem Res Serum Proteomics Identified TAFI as a Potential Molecule Facilitating the Migration of Peripheral Monocytes to Damaged White Matter During Chronic Cerebral Hypoperfusion | ||
Int J Biol Macromol Multi-omics analysis reveals the pre-protective mechanism of Dendrobium flexicaule polysaccharide against alcohol-induced gastric mucosal injury |
