• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CPEB1 Recombinant antibody

CPEB1 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

6I9

Cat no : 82785-1-RR

Print datasheet

Synonyms

CPE-binding protein 1, CPEB, CPE BP1, CPE binding protein 1, CEBP

验证数据展示

  • IF Staining of HeLa using 82785-1-RR

    IF Staining of HeLa using 82785-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CPEB1 antibody (82785-1-RR, Clone: 6I9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • Affinity and Kinetic Characterization of 82785-1-RR

    Affinity and Kinetic Characterization of 82785-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82785-1-RR against Human CPEB1 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82785-1-RR targets CPEB1 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen CPEB1 fusion protein Ag3994 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cytoplasmic polyadenylation element binding protein 1
Synonyms CPE-binding protein 1, CPEB, CPE BP1, CPE binding protein 1, CEBP
Calculated Molecular Weight 486 aa, 54 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC035348
Gene Symbol CPEB1
Gene ID (NCBI) 64506
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BZB8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CPEB1, also named as CEBP, CPEB and CPE-BP1, contains two RRM (RNA recognition motif) domains and belongs to the RRM CPEB family. It is a sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. CPEB1 regulated translation is a key process involved in INS signaling.

实验方案

Product Specific Protocols
IF protocol for CPEB1 antibody 82785-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols