验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, A549 cells, rat brain tissue |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18149-1-AP targets CPLX2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | CPLX2 fusion protein Ag12838 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | complexin 2 |
| 别名 | CPX II, CPX 2, CPLX 2, Complexin-2, 921L |
| 计算分子量 | 134 aa, 15 kDa |
| 观测分子量 | 18-20 kDa |
| GenBank蛋白编号 | BC093706 |
| 基因名称 | CPLX2 |
| Gene ID (NCBI) | 10814 |
| RRID | AB_2276580 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6PUV4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Neuronal specific complexin 1 (CPLX1) has inhibitory and stimulatory effects on exocytosis by clamping trans-SNARE complexes in a prefusion state and promoting conformational changes to facilitate membrane fusion following cell stimulation. Complexin2 (CPLX2) is a pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals, it is downregulated in schizophrenic patients suffering from depression, animal models of depression and neurological disorders such as Huntington's disease in which depression is a major symptom.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CPLX2 antibody 18149-1-AP | Download protocol |
| IHC protocol for CPLX2 antibody 18149-1-AP | Download protocol |
| IF protocol for CPLX2 antibody 18149-1-AP | Download protocol |
| IP protocol for CPLX2 antibody 18149-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Life Sci Geniposide improves depression-like behavior in prenatal stress male offspring through restoring HPA axis- and glucocorticoid receptor-associated dysfunction | ||
Free Radic Biol Med NOX2 Deficiency Ameliorates Cerebral Injury by Reduction of Complexin II-mediated Glutamate Excitotoxicity in Experimental Stroke.
| ||
Behav Brain Res Widespread cerebellar transcriptome changes in Ts65Dn down syndrome mouse model after lifelong running. | ||
Cancer Biomark Complexin-2 (CPLX2) as a potential prognostic biomarker in human lung high grade neuroendocrine tumors. | ||
bioRxiv Role of Complexin 2 in the regulation of hormone secretion from the islet of Langerhans
| ||
Neural Regen Res Nicotinamide adenine dinucleotide treatment confers resistance to neonatal ischemia and hypoxia: effects on neurobehavioral phenotypes |