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验证数据展示

  • WB analysis of mouse brain using 20249-1-AP

    WB analysis of mouse brain using 20249-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20249-1-AP (CPLX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 20249-1-AP

    IHC staining of human brain using 20249-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain slide using 20249-1-AP (CPLX2 Antibody) at dilution of 1:50.

CPLX2 Polyclonal antibody

CPLX2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20249-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

921 L, complexin 2, Complexin II, CPLX2, CPX 2, CPX II, CPX2, Hfb1, Synaphin 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20249-1-AP targets CPLX2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 complexin 2
别名 921 L, complexin 2, Complexin II, CPLX2, CPX 2, CPX II, CPX2, Hfb1, Synaphin 1
计算分子量 15 kDa
观测分子量 20 kDa
GenBank蛋白编号NM_006650
基因名称 CPLX2
Gene ID (NCBI) 10814
RRIDAB_11232598
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6PUV4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Neuronal specific complexin 1 (CPLX1) has inhibitory and stimulatory effects on exocytosis by clamping trans-SNARE complexes in a prefusion state and promoting conformational changes to facilitate membrane fusion following cell stimulation. Complexin2 (CPLX2) is a pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals, it is downregulated in schizophrenic patients suffering from depression, animal models of depression and neurological disorders such as Huntington's disease in which depression is a major symptom. This antibody is expected to specifically recogize CPLX2 but not other complexins.

实验方案

Product Specific Protocols
WB protocol for CPLX2 antibody 20249-1-APDownload protocol
IHC protocol for CPLX2 antibody 20249-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols