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验证数据展示

  • WB analysis of mouse brain using 20249-1-AP

    WB analysis of mouse brain using 20249-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20249-1-AP (CPLX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 20249-1-AP

    IHC staining of human brain using 20249-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain slide using 20249-1-AP (CPLX2 Antibody) at dilution of 1:50.

CPLX2 Polyclonal antibody

CPLX2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20249-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

921 L, complexin 2, Complexin II, CPLX2, CPX 2, CPX II, CPX2, Hfb1, Synaphin 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20249-1-AP targets CPLX2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name complexin 2
Synonyms 921 L, complexin 2, Complexin II, CPLX2, CPX 2, CPX II, CPX2, Hfb1, Synaphin 1
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberNM_006650
Gene Symbol CPLX2
Gene ID (NCBI) 10814
RRIDAB_11232598
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6PUV4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Neuronal specific complexin 1 (CPLX1) has inhibitory and stimulatory effects on exocytosis by clamping trans-SNARE complexes in a prefusion state and promoting conformational changes to facilitate membrane fusion following cell stimulation. Complexin2 (CPLX2) is a pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals, it is downregulated in schizophrenic patients suffering from depression, animal models of depression and neurological disorders such as Huntington's disease in which depression is a major symptom. This antibody is expected to specifically recogize CPLX2 but not other complexins.

实验方案

Product Specific Protocols
WB protocol for CPLX2 antibody 20249-1-APDownload protocol
IHC protocol for CPLX2 antibody 20249-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols