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CPLX4 Polyclonal antibody

CPLX4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21222-1-AP

Print datasheet

Synonyms

complexin 4, Complexin IV, CPLX4, CPX IV, CPXIV

验证数据展示

  • IHC staining of mouse eye using 21222-1-AP

    IHC staining of mouse eye using 21222-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21222-1-AP (CPLX4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of Y79 using 21222-1-AP

    WB analysis of Y79 using 21222-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 21222-1-AP (CPLX4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of Y79 using 21222-1-AP

    IP experiment of Y79 using 21222-1-AP

    IP result of anti-CPLX4 (IP:21222-1-AP, 3ug; Detection:21222-1-AP 1:1000) with Y79 cells lysate 960ug.

  • WB analysis of Y79 using 21222-1-AP

    WB analysis of Y79 using 21222-1-AP

    WB result of CPLX4 antibody (21222-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPLX4 transfected Y79 cells.

经过测试的应用

Positive WB detected inY79 cells
Positive IP detected inY79 cells
Positive IHC detected inmouse eye tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21222-1-AP targets CPLX4 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CPLX4 fusion protein Ag15661 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name complexin 4
Synonyms complexin 4, Complexin IV, CPLX4, CPX IV, CPXIV
Calculated Molecular Weight 160 aa, 18 kDa
Observed Molecular Weight 21-23 kDa
GenBank Accession NumberBC106887
Gene Symbol CPLX4
Gene ID (NCBI) 339302
RRIDAB_10733348
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z7G2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CPLX4 antibody 21222-1-APDownload protocol
IHC protocol for CPLX4 antibody 21222-1-APDownload protocol
IP protocol for CPLX4 antibody 21222-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols