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验证数据展示

  • WB analysis of Y79 using 21222-1-AP

    WB analysis of Y79 using 21222-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 21222-1-AP (CPLX4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of rat eye using 21222-1-AP

    IF Staining of rat eye using 21222-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using CPLX4 antibody (21222-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of Y79 using 21222-1-AP

    IP experiment of Y79 using 21222-1-AP

    IP result of anti-CPLX4 (IP:21222-1-AP, 3ug; Detection:21222-1-AP 1:1000) with Y79 cells lysate 960ug.

  • WB analysis of Y79 using 21222-1-AP

    WB analysis of Y79 using 21222-1-AP

    WB result of CPLX4 antibody (21222-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPLX4 transfected Y79 cells.

  • IHC staining of mouse eye using 21222-1-AP

    IHC staining of mouse eye using 21222-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21222-1-AP (CPLX4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat eye using 21222-1-AP

    IHC staining of rat eye using 21222-1-AP

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 21222-1-AP (CPLX4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

CPLX4 Polyclonal antibody

CPLX4 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 21222-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IP, ELISA

别名

complexin 4, Complexin IV, Complexin-4, CPX IV, CPXIV

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inY79 cells
Positive IP detected inY79 cells
Positive IHC detected inmouse eye tissue, human brain tissue, rat eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat eye tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21222-1-AP targets CPLX4 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CPLX4 fusion protein Ag15661 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 complexin 4
别名 complexin 4, Complexin IV, Complexin-4, CPX IV, CPXIV
计算分子量 160 aa, 18 kDa
观测分子量 21-23 kDa
GenBank蛋白编号BC106887
基因名称 CPLX4
Gene ID (NCBI) 339302
RRIDAB_10733348
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z7G2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CPLX4 antibody 21222-1-APDownload protocol
IHC protocol for CPLX4 antibody 21222-1-APDownload protocol
IF protocol for CPLX4 antibody 21222-1-APDownload protocol
IP protocol for CPLX4 antibody 21222-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols