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CPN1 Polyclonal antibody

CPN1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31940-1-AP

Print datasheet

Synonyms

Kininase-1, Kininase 1, EC:3.4.17.3, CPN, Arginine carboxypeptidase

验证数据展示

  • WB analysis using 31940-1-AP

    WB analysis using 31940-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31940-1-AP (CPN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman plasma

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31940-1-AP targets CPN1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen CPN1 fusion protein Ag34806 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name carboxypeptidase N, polypeptide 1
Synonyms Kininase-1, Kininase 1, EC:3.4.17.3, CPN, Arginine carboxypeptidase
Calculated Molecular Weight 458 aa, 52 kDa
Observed Molecular Weight 40~50 kDa
GenBank Accession NumberBC027897
Gene Symbol CPN1
Gene ID (NCBI) 1369
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP15169
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human carboxypeptidase N (CPN), a member of the CPN/E subfamily of "regulatory" metallo-carboxypeptidases, is an extracellular glycoprotein synthesized in the liver and secreted into the blood, where it controls the activity of vasoactive peptide hormones, growth factors and cytokines by specifically removing C-terminal basic residues. Normally, CPN circulates in blood plasma as a hetero-tetramer consisting of two 83 kDa (CPN2) domains each flanked by a 48 to 55 kDa catalytic (CPN1) domain. The C terminal region of the CPN1 subunit differs from all other family members in that it contains numerous basic residues. Proteolysis at these sites apparently happens either constitutively in the blood or during processing and secretion from the liver.

实验方案

Product Specific Protocols
WB protocol for CPN1 antibody 31940-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols