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CPNE3 Recombinant antibody

CPNE3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

240871E5

Cat no : 83820-5-RR

Print datasheet

Synonyms

CPN3, copineIII, Copine-3, Copine III, Copine 3

验证数据展示

  • WB analysis using 83820-5-RR

    WB analysis using 83820-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83820-5-RR (CPNE3 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 83820-5-RR

    IP experiment of HepG2 using 83820-5-RR

    IP result of anti-CPNE3 (IP:83820-5-RR, 4ug; Detection:83820-5-RR 1:2000) with HepG2 cells lysate 1600 ug.

  • IF Staining of A431 using 83820-5-RR

    IF Staining of A431 using 83820-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CPNE3 antibody (83820-5-RR, Clone: 240871E5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 83820-5-RR

    FC experiment of HepG2 using 83820-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CPNE3 Recombinant antibody (83820-5-RR, Clone:240871E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83820-5-RR

    Affinity and Kinetic Characterization of 83820-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83820-5-RR against Human CPNE3 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inY79 cells, HepG2 cells, A431 cells, A549 cells, HeLa cells, HuH-7 cells
Positive IP detected inHepG2 cells
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83820-5-RR is CPNE3 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), IP, ELISA Application Description
Tested Reactivity human
Immunogen CPNE3 fusion protein Ag1634 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name copine III
Synonyms CPN3, copineIII, Copine-3, Copine III, Copine 3
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC036242
Gene Symbol CPNE3
Gene ID (NCBI) 8895
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO75131
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Copines are a family of evolutionarily conserved calcium-dependent phospholipid-binding proteins (PMID: 9430674). They contain two Ca(2+)- and phospholipid-binding domains known as C2 domains. Copines are potentially involved in regulating membrane trafficking and in protein-protein interactions. Copine III (CPNE3) has also been identified as a ligand of ERBB2 and plays an important role in various carcinogenesis. The high expression of CPNE3 is an adverse prognostic biomarker for acute myeloid leukemia (PMID: 28670859).

实验方案

Product Specific Protocols
WB protocol for CPNE3 antibody 83820-5-RRDownload protocol
IF protocol for CPNE3 antibody 83820-5-RRDownload protocol
IP protocol for CPNE3 antibody 83820-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols