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CPOX Polyclonal antibody

CPOX Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16724-1-AP

Print datasheet

Synonyms

Coprogen oxidase, Coproporphyrinogen oxidase, Coproporphyrinogenase, COX, CPO, CPOX, CPX, HCP

验证数据展示

  • WB analysis using 16724-1-AP

    WB analysis using 16724-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16724-1-AP

    IHC staining of human liver using 16724-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16724-1-AP (CPOX antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver using 16724-1-AP

    IHC staining of human liver using 16724-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16724-1-AP (CPOX antibody) at dilution of 1:100 (under 40x lens).

  • WB analysis of K-562 using 16724-1-AP

    WB analysis of K-562 using 16724-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 16724-1-AP

    IP experiment of HepG2 using 16724-1-AP

    IP result of anti-CPOX (IP:16724-1-AP, 4ug; Detection:16724-1-AP 1:1000) with HepG2 cells lysate 1800ug.

经过测试的应用

Positive WB detected inHepG2 cells, human liver tissue, K-562 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16724-1-AP targets CPOX in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CPOX fusion protein Ag10164 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coproporphyrinogen oxidase
Synonyms Coprogen oxidase, Coproporphyrinogen oxidase, Coproporphyrinogenase, COX, CPO, CPOX, CPX, HCP
Calculated Molecular Weight 454 aa, 50 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC017210
Gene Symbol CPOX
Gene ID (NCBI) 1371
RRIDAB_2084231
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP36551
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CPOX(Coproporphyrinogen-III oxidase, mitochondrial) is also named as CPO and CPX. Human CPO is a 76 kDa protein that is active as a homodimer in the mitochondrial intermembrane pace(PMID:16159891).Mutations in human CPO gene predict the clinical outcome of the disease, with either hepatic hereditary coproporphyria or hematological manifestations of erythropoietic harderoporphyria.CPOX has a transit peptide of 110 aa and the chain contains 344 aa.

实验方案

Product Specific Protocols
WB protocol for CPOX antibody 16724-1-APDownload protocol
IHC protocol for CPOX antibody 16724-1-APDownload protocol
IP protocol for CPOX antibody 16724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols