验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, human liver tissue, K-562 cells, mouse liver tissue, rat liver tissue |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human liver tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16724-1-AP targets CPOX in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IP, IHC, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | CPOX fusion protein Ag10164 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | coproporphyrinogen oxidase |
别名 | Coprogen oxidase, Coproporphyrinogen oxidase, Coproporphyrinogenase, COX, CPO, CPOX, CPX, HCP |
计算分子量 | 454 aa, 50 kDa |
观测分子量 | 36 kDa |
GenBank蛋白编号 | BC017210 |
基因名称 | CPOX |
Gene ID (NCBI) | 1371 |
RRID | AB_2084231 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P36551 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CPOX(Coproporphyrinogen-III oxidase, mitochondrial) is also named as CPO and CPX. Human CPO is a 76 kDa protein that is active as a homodimer in the mitochondrial intermembrane pace(PMID:16159891).Mutations in human CPO gene predict the clinical outcome of the disease, with either hepatic hereditary coproporphyria or hematological manifestations of erythropoietic harderoporphyria.CPOX has a transit peptide of 110 aa and the chain contains 344 aa.
实验方案
Product Specific Protocols | |
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WB protocol for CPOX antibody 16724-1-AP | Download protocol |
IHC protocol for CPOX antibody 16724-1-AP | Download protocol |
IP protocol for CPOX antibody 16724-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |