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CPPED1 Polyclonal antibody

CPPED1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20628-1-AP

Print datasheet

Synonyms

CPPED1, CSTP1

验证数据展示

  • WB analysis of SMMC-7721 using 20628-1-AP

    WB analysis of SMMC-7721 using 20628-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 20628-1-AP (CPPED1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 20628-1-AP

    IHC staining of human liver cancer using 20628-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20628-1-AP (CPPED1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 20628-1-AP

    IHC staining of human liver cancer using 20628-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20628-1-AP (CPPED1 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inSMMC-7721 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20628-1-AP is CPPED1 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen CPPED1 fusion protein Ag14572 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calcineurin-like phosphoesterase domain containing 1
Synonyms CPPED1, CSTP1
Calculated Molecular Weight 314 aa, 36 kDa
Observed Molecular Weight 28-35 kDa
GenBank Accession NumberBC006289
Gene Symbol CPPED1
Gene ID (NCBI) 55313
RRIDAB_2878711
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRF8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CPPED1, also named as CSTP1, plays an inhibitory role in glucose uptake by adipocytes. It has some isoforms with MW 20 kDa and 28-35 kDa.

实验方案

Product Specific Protocols
WB protocol for CPPED1 antibody 20628-1-APDownload protocol
IHC protocol for CPPED1 antibody 20628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols