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验证数据展示

  • WB analysis of HepG2 using 24513-1-AP

    WB analysis of HepG2 using 24513-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24513-1-AP (CPS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 24513-1-AP

    IHC staining of human liver cancer using 24513-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24513-1-AP (CPS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CPS1 antibody included
  • WB analysis using 24513-1-AP

    WB analysis using 24513-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24513-1-AP (CPS1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

CPS1 Polyclonal antibody

CPS1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24513-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CPS1, CPSase I

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, rat liver tissue, mouse liver tissue, L02 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CPS1 Ready-To-Use IHC Kit. CPS1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24513-1-AP targets CPS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CPS1 fusion protein Ag21605 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carbamoyl-phosphate synthetase 1, mitochondrial
别名 CPS1, CPSase I
计算分子量 1500 aa, 165 kDa
观测分子量140-165 kDa
GenBank蛋白编号BC140943
基因名称 CPS1
Gene ID (NCBI) 1373
RRIDAB_2879583
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP31327
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Carbamoyl phosphate synthetase 1 (CPS1) is the first rate-limiting enzyme in the urea cycle, converting ammonium into carbamoyl phosphate, and plays an intricate role in arginine metabolism and pyrimidine metabolism (PMID: 28376202). CPS1 expression is liver specific and suppressed in HCC cells and tumor tissues at the transcriptional level(PMID: 21281797). CPS1 gene comprises 38 exons that encode a polypeptide of 1500 amino acids and has a molecular weight of 165 kDa.

实验方案

Product Specific Protocols
WB protocol for CPS1 antibody 24513-1-APDownload protocol
IHC protocol for CPS1 antibody 24513-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols