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CPS1 Recombinant antibody, PBS Only

CPS1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241198F1

Cat no : 84277-1-PBS

Print datasheet

Synonyms

EC:6.3.4.16, CPSase I, Carbamoyl-phosphate synthetase I, Carbamoyl-phosphate synthase [ammonia], mitochondrial, 241198F1

验证数据展示

  • WB analysis of HeLa using 84277-1-RR (same clone as 84277-1-PBS)

    WB analysis of HeLa using 84277-1-RR (same clone as 84277-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 84277-1-RR (CPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84277-1-PBS in a different storage buffer formulation.

  • WB analysis using 84277-1-RR (same clone as 84277-1-PBS)

    WB analysis using 84277-1-RR (same clone as 84277-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84277-1-RR (CPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84277-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84277-1-RR (same clone as 84277-1-PBS)

    IF Staining of HepG2 using 84277-1-RR (same clone as 84277-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CPS1 antibody (84277-1-RR, Clone: 241198F1 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84277-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CPS1 Ready-To-Use IHC Kit. CPS1 primary antibody included.

产品信息

84277-1-PBS targets CPS1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name carbamoyl-phosphate synthetase 1, mitochondrial
Synonyms EC:6.3.4.16, CPSase I, Carbamoyl-phosphate synthetase I, Carbamoyl-phosphate synthase [ammonia], mitochondrial, 241198F1
Calculated Molecular Weight 165 kDa
Observed Molecular Weight140-165 kDa
GenBank Accession NumberBC140943
Gene Symbol CPS1
Gene ID (NCBI) 1373
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP31327
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Carbamoyl phosphate synthetase 1 (CPS1) is the first rate-limiting enzyme in the urea cycle, converting ammonium into carbamoyl phosphate, and plays an intricate role in arginine metabolism and pyrimidine metabolism (PMID: 28376202). CPS1 expression is liver specific and suppressed in HCC cells and tumor tissues at the transcriptional level(PMID: 21281797). CPS1 gene comprises 38 exons that encode a polypeptide of 1500 amino acids and has a molecular weight of 165 kDa.