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CPSF2 Polyclonal antibody

CPSF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17739-1-AP

Print datasheet

Synonyms

KIAA1367, CPSF100, CPSF 100 kDa subunit

验证数据展示

  • WB analysis of HeLa using 17739-1-AP

    WB analysis of HeLa using 17739-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17739-1-AP (CPSF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 17739-1-AP

    IHC staining of human brain using 17739-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17739-1-AP (CPSF2 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with CPSF2 antibody included
  • IHC staining of human brain using 17739-1-AP

    IHC staining of human brain using 17739-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17739-1-AP (CPSF2 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with CPSF2 antibody included
  • IP experiment of HeLa using 17739-1-AP

    IP experiment of HeLa using 17739-1-AP

    IP result of anti-CPSF2 (IP:17739-1-AP, 4ug; Detection:17739-1-AP 1:1000) with HeLa cells lysate 4000ug.

  • IF Staining of HepG2 using 17739-1-AP

    IF Staining of HepG2 using 17739-1-AP

    Immunofluorescent analysis of HepG2 cells using 17739-1-AP (CPSF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, human brain tissue, mouse brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CPSF2 Ready-To-Use IHC Kit. CPSF2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17739-1-AP targets CPSF2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CPSF2 fusion protein Ag11950 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cleavage and polyadenylation specific factor 2, 100kDa
Synonyms KIAA1367, CPSF100, CPSF 100 kDa subunit
Calculated Molecular Weight 782 aa, 88 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC070095
Gene Symbol CPSF2
Gene ID (NCBI) 53981
RRIDAB_2084368
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2I0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CPSF2 antibody 17739-1-APDownload protocol
IHC protocol for CPSF2 antibody 17739-1-APDownload protocol
IF protocol for CPSF2 antibody 17739-1-APDownload protocol
IP protocol for CPSF2 antibody 17739-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanCoIP

Neuron

Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration.

Authors - Geneva R LaForce
View Article
humanWB

Cell Discov

RBBP6 maintains glioblastoma stem cells through CPSF3-dependent alternative polyadenylation

Authors - Peng Lin
View Article