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验证数据展示

  • WB analysis of HeLa using 17739-1-AP

    WB analysis of HeLa using 17739-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17739-1-AP (CPSF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 17739-1-AP

    IHC staining of human brain using 17739-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17739-1-AP (CPSF2 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with CPSF2 antibody included
  • IHC staining of human brain using 17739-1-AP

    IHC staining of human brain using 17739-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17739-1-AP (CPSF2 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with CPSF2 antibody included
  • IP experiment of HeLa using 17739-1-AP

    IP experiment of HeLa using 17739-1-AP

    IP result of anti-CPSF2 (IP:17739-1-AP, 4ug; Detection:17739-1-AP 1:1000) with HeLa cells lysate 4000ug.

  • IF Staining of HepG2 using 17739-1-AP

    IF Staining of HepG2 using 17739-1-AP

    Immunofluorescent analysis of HepG2 cells using 17739-1-AP (CPSF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

CPSF2 Polyclonal antibody

CPSF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 17739-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

KIAA1367, CPSF100, CPSF 100 kDa subunit

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, human brain tissue, mouse brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CPSF2 Ready-To-Use IHC Kit. CPSF2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17739-1-AP targets CPSF2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 CPSF2 fusion protein Ag11950 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cleavage and polyadenylation specific factor 2, 100kDa
别名 KIAA1367, CPSF100, CPSF 100 kDa subunit
计算分子量 782 aa, 88 kDa
观测分子量 100 kDa
GenBank蛋白编号BC070095
基因名称 CPSF2
Gene ID (NCBI) 53981
RRIDAB_2084368
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P2I0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CPSF2 antibody 17739-1-APDownload protocol
IHC protocol for CPSF2 antibody 17739-1-APDownload protocol
IF protocol for CPSF2 antibody 17739-1-APDownload protocol
IP protocol for CPSF2 antibody 17739-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanCoIP

Neuron

Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration.

Authors - Geneva R LaForce
View Article
humanWB

Cell Discov

RBBP6 maintains glioblastoma stem cells through CPSF3-dependent alternative polyadenylation

Authors - Peng Lin
View Article