验证数据展示
经过测试的应用
| Positive WB detected in | mouse colon tissue, mouse spleen tissue |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
15860-1-AP targets INTS11 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | INTS11 fusion protein Ag8365 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cleavage and polyadenylation specific factor 3-like |
| 别名 | INT11, Cleavage and polyadenylation-specific factor 3-like protein, CPSF3L, CPSF3-like protein, EC:3.1.27.- |
| 计算分子量 | 600 aa, 68 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC007978 |
| 基因名称 | INT11 |
| Gene ID (NCBI) | 54973 |
| RRID | AB_2084537 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5TA45 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Integrator complex subunit 11 (INT11) is a protein that in humans is encoded by the INTS11 gene. INTS11, also known as Cleavage and Polyadenylation Specific Factor 3-Like (CPSF3L), contains a metallo-β-lactamase and β-CASP domain that, together, coordinate two zinc ions within the active site to promote its RNA endonuclease activity. INTS11 is the catalytic subunit of the Integrator (INT) complex, which is crucial for the biogenesis of small nuclear RNAs and enhancer RNAs. INT11 has 5 isoforms with the molecular mass of 56 and 65-68 kDa (PMID: 34516911, PMID: 37054711).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for INTS11 antibody 15860-1-AP | Download protocol |
| IF protocol for INTS11 antibody 15860-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
