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- KD/KO Validated
CPSF4 Polyclonal antibody
CPSF4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HepG2 cells, HeLa cells, HEK-293 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15023-1-AP targets CPSF4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, pig |
| Immunogen | CPSF4 fusion protein Ag7103 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cleavage and polyadenylation specific factor 4, 30kDa |
| Synonyms | Neb 1, NAR, CPSF30, CPSF 30 kDa subunit, Cleavage and polyadenylation specificity factor subunit 4 |
| Calculated Molecular Weight | 30 kDa |
| Observed Molecular Weight | 27-30 kDa |
| GenBank Accession Number | BC003101 |
| Gene Symbol | CPSF4 |
| Gene ID (NCBI) | 10898 |
| RRID | AB_2084544 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95639 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The mature 3-prime ends of cellular mRNAs are producted by endonucleolytic cleavage of the primary transcripts, followed by polyadenylation of the upstream cleavage products. The cleavage and polyadenylation specificity factor (CPSF) has a essential role in pre-mRNA 3-prime end processing in that it is required in both the cleavage step and subsequent poly(A) addition [PMID: 9651582]. CPSF4 is a component of the cleavage and polyadenylation specificity factor (CPSF) complex, whose other components are CPSF1, CPSF2, CPSF3 and FIP1L1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CPSF4 antibody 15023-1-AP | Download protocol |
| IHC protocol for CPSF4 antibody 15023-1-AP | Download protocol |
| IF protocol for CPSF4 antibody 15023-1-AP | Download protocol |
| IP protocol for CPSF4 antibody 15023-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation | ||
Mol Oncol CPSF4 activates telomerase reverse transcriptase and predicts poor prognosis in human lung adenocarcinomas.
| ||
Cancer Lett Cleavage and polyadenylation specific factor 4 targets NF-κB/cyclooxygenase-2 signaling to promote lung cancer growth and progression.
| ||
Mol Oncol CREB-binding protein regulates lung cancer growth by targeting MAPK and CPSF4 signaling pathway. | ||
Int J Biol Macromol Key pituitary miRNAs mediate the expression of pig GHRHR splice variants by regulating splice factors. | ||
Biochim Biophys Acta Mol Cell Res Cleavage and polyadenylation specific factor 4 promotes colon cancer progression by transcriptionally activating hTERT. |
