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  • Featured Product
  • KD/KO Validated

CPSF6 Polyclonal antibody

CPSF6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15489-1-AP

Print datasheet

Synonyms

CPSF 68 kDa subunit, Cleavage factor Im complex 68 kDa subunit, Cleavage and polyadenylation specificity factor subunit 6, Cleavage and polyadenylation specificity factor 68 kDa subunit, CFIm68

验证数据展示

  • WB analysis of HeLa using 15489-1-AP

    WB analysis of HeLa using 15489-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 15489-1-AP

    IHC staining of human kidney using 15489-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15489-1-AP (CPSF6 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with CPSF6 antibody included
  • IHC staining of human kidney using 15489-1-AP

    IHC staining of human kidney using 15489-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15489-1-AP (CPSF6 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with CPSF6 antibody included
  • IP experiment of HeLa using 15489-1-AP

    IP experiment of HeLa using 15489-1-AP

    IP result of anti-CPSF6 (IP:15489-1-AP, 4ug; Detection:15489-1-AP 1:300) with HeLa cells lysate 3200ug.

  • WB analysis using 15489-1-AP

    WB analysis using 15489-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 15489-1-AP

    IHC staining of human liver using 15489-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15489-1-AP (CPSF6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CPSF6 antibody included
  • IHC staining of human liver using 15489-1-AP

    IHC staining of human liver using 15489-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15489-1-AP (CPSF6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CPSF6 antibody included
  • IF Staining of SW480 using 15489-1-AP

    IF Staining of SW480 using 15489-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SW480 cells using CPSF6 antibody (15489-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, PC-3 cells, MDA-MB-231 cells, SW480 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver tissue, human heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSW480 cells
Planning an IHC experiment? We recommend our IHCeasy CPSF6 Ready-To-Use IHC Kit. CPSF6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15489-1-AP targets CPSF6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen CPSF6 fusion protein Ag7852 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cleavage and polyadenylation specific factor 6, 68kDa
Synonyms CPSF 68 kDa subunit, Cleavage factor Im complex 68 kDa subunit, Cleavage and polyadenylation specificity factor subunit 6, Cleavage and polyadenylation specificity factor 68 kDa subunit, CFIm68
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 55-68 kDa
GenBank Accession NumberBC000714
Gene Symbol CPSF6
Gene ID (NCBI) 11052
RRIDAB_10694140
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16630
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The binding of Cleavage factor Im (CFIM), also known as CPSF6, to the pre-mRNA is one of the earliest steps in the assembly of the cleavage and polyadenylation machinery and facilitates the recruitment of other processing factors. CFIM is required for the first step in pre-mRNA 3′-end processing and can be reconstituted in vitro from its heterologously expressed 25- and 68-kDa subunits. It involved in RNA binding, protein-protein interactions, and subcellular localization [PMID:15169763]. In addition, it is a pre-mRNA processing protein that dynamically shuttles between the nucleus and the cytoplasm and contains a C-terminal nuclear-targeting arginine/serine-rich (RS-) domain of the type bound by TNPO3[PMID:15169763,19864460].

实验方案

Product Specific Protocols
WB protocol for CPSF6 antibody 15489-1-APDownload protocol
IHC protocol for CPSF6 antibody 15489-1-APDownload protocol
IF protocol for CPSF6 antibody 15489-1-APDownload protocol
IP protocol for CPSF6 antibody 15489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

A Quantitative Genetic Interaction Map of HIV Infection.

Authors - David E Gordon
View Article
humanWB

PLoS Pathog

In Vivo Functions of CPSF6 for HIV-1 as Revealed by HIV-1 Capsid Evolution in HLA-B27-Positive Subjects.

Authors - Matthew S Henning
View Article
humanWB

Elife

Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2.

Authors - Melissa Kane
View Article
humanWB

J Virol

Cell type-dependent escape of capsid inhibitors by simian immunodeficiency virus SIVcpz.

Authors - Augustin Penda Twizerimana
View Article
humanWB

iScience

Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.

Authors - Mio Iwasaki
View Article
humanWB

J Virol

Roles of capsid-interacting host factors in multimodal inhibition of HIV-1 by PF74.

Authors - Akatsuki Saito
  • KD Validated
View Article