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  • Featured Product
  • KD/KO Validated

CPT1A Monoclonal antibody, PBS Only

CPT1A Monoclonal Antibody for WB,IP,IHC,FC,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB,IP,IHC,FC,ELISA

Conjugate

Unconjugated

CloneNo.

5D8G9

Cat No : 66039-1-PBS

Print datasheet

Synonyms

CPT I, CPT1, CPT1 L, CPT1A, CPTI L, L CPT1

验证数据展示

  • WB analysis of A549 using 66039-1-Ig (same clone as 66039-1-PBS)

    WB analysis of A549 using 66039-1-Ig (same clone as 66039-1-PBS)

    WB result of CPT1A antibody (66039-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPT1A transfected A549 cells. This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66039-1-Ig (same clone as 66039-1-PBS)

    IHC staining of human breast cancer using 66039-1-Ig (same clone as 66039-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66039-1-Ig (CPT1A antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CPT1A antibody included
  • IHC staining of human breast cancer using 66039-1-Ig (same clone as 66039-1-PBS)

    IHC staining of human breast cancer using 66039-1-Ig (same clone as 66039-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66039-1-Ig (CPT1A antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CPT1A antibody included
  • IP experiment of MCF-7 using 66039-1-Ig (same clone as 66039-1-PBS)

    IP experiment of MCF-7 using 66039-1-Ig (same clone as 66039-1-PBS)

    IP result of anti-CPT1A (IP:66039-1-Ig, 4ug; Detection:66039-1-Ig 1:500) with MCF-7 cells lysate 1200ug. This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66039-1-Ig (same clone as 66039-1-PBS)

    FC experiment of HeLa using 66039-1-Ig (same clone as 66039-1-PBS)

    1X10^6 HeLa cells were stained with 0.2ug CPT1A antibody (66039-1-Ig, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CPT1A Ready-To-Use IHC Kit. CPT1A primary antibody included.

产品信息

66039-1-PBS targets CPT1A in WB, IP, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB,IP,IHC,FC,ELISA
Tested Reactivity human, mouse, rat
Immunogen CPT1A fusion protein Ag7745 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name carnitine palmitoyltransferase 1A (liver)
Synonyms CPT I, CPT1, CPT1 L, CPT1A, CPTI L, L CPT1
Calculated Molecular Weight 88 kDa
Observed Molecular Weight 86 kDa
GenBank Accession NumberBC000185
Gene Symbol CPT1A
Gene ID (NCBI) 1374
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50416
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.