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验证数据展示

  • WB analysis using 85013-4-RR

    WB analysis using 85013-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85013-4-RR (CPT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85013-4-RR

    Affinity and Kinetic Characterization of 85013-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85013-4-RR against Human CPT2 were performed. The affinity constant is 0.261 nM.

CPT2 Recombinant antibody

CPT2 Recombinant Antibody for WB, ELISA
Cat No. 85013-4-RR

产品说明书

CloneNo. 242381C5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

EC:2.3.1.21, CPTASE, CPT II, carnitine palmitoyltransferase 2, Carnitine O-palmitoyltransferase 2, mitochondrial

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, MCF-7 cells, HepG2 cells, T-47D cells, LNCaP cells, mouse liver tissue, rat liver tissue
Planning an IHC experiment? We recommend our IHCeasy CPT2 Ready-To-Use IHC Kit. CPT2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85013-4-RR targets CPT2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CPT2 fusion protein Ag24897 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 carnitine palmitoyltransferase 2
别名 EC:2.3.1.21, CPTASE, CPT II, carnitine palmitoyltransferase 2, Carnitine O-palmitoyltransferase 2, mitochondrial
计算分子量 74 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC002445
基因名称 CPT2
Gene ID (NCBI) 1376
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP23786
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Carnitine palmitoyltransferase-1 and -2 (CPT1 and CPT2) are two genetically distinct mitochondrial membrane bound enzymes and play critical role in the regulation of FAO in normal cells. CPT2 is an ubiquitous protein and locates in the inner membrane of mitochondrial (PMID: 29437870). CPT2 is frequently down-regulated in primary ovarian serous carcinomas, which is significantly correlated with poor survival of ovarian cancer patients (PMID: 33486313). Down-regulation of CPT2 was a major cause of acylcarnitine accumulation and a common feature in mouse models of obesity- and NASH-driven HCC and human SH-HCC (PMID: 29872321).

实验方案

Product Specific Protocols
WB protocol for CPT2 antibody 85013-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols