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验证数据展示

  • WB analysis of HEK-293 using 12548-1-AP

    WB analysis of HEK-293 using 12548-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 12548-1-AP

    IP experiment of HEK-293 using 12548-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.

  • IHC staining of human liver cancer using 12548-1-AP

    IHC staining of human liver cancer using 12548-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 12548-1-AP (CPVL antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 12548-1-AP

    IHC staining of human liver cancer using 12548-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 12548-1-AP (CPVL antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HepG2 using 12548-1-AP

    IF Staining of HepG2 using 12548-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 12548-1-AP (CPVL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 12548-1-AP

    FC experiment of HepG2 using 12548-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CPVL Polyclonal antibody (12548-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CPVL Polyclonal antibody

CPVL Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 12548-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

别名

VCP like protein, UNQ197/PRO223, PSEC0124, hVLP, EC:3.4.16.-

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, human heart tissue, human kidney tissue, human liver tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

12548-1-AP targets CPVL in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 CPVL fusion protein Ag3398 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carboxypeptidase, vitellogenic-like
别名 VCP like protein, UNQ197/PRO223, PSEC0124, hVLP, EC:3.4.16.-
计算分子量 476 aa, 54 kDa
观测分子量 54 kDa
GenBank蛋白编号BC016838
基因名称 CPVL
Gene ID (NCBI) 54504
RRIDAB_2084865
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H3G5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CPVL antibody 12548-1-APDownload protocol
IHC protocol for CPVL antibody 12548-1-APDownload protocol
IF protocol for CPVL antibody 12548-1-APDownload protocol
IP protocol for CPVL antibody 12548-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Pharmacol

Dissecting gastric cancer heterogeneity and exploring therapeutic strategies using bulk and single-cell transcriptomic analysis and experimental validation of tumor microenvironment and metabolic interplay

Authors - XianTao Lin
View Article