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CPVL Polyclonal antibody

CPVL Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 12548-1-AP

Print datasheet

Synonyms

VCP like protein, UNQ197/PRO223, PSEC0124, hVLP, EC:3.4.16.-

验证数据展示

  • WB analysis of HEK-293 using 12548-1-AP

    WB analysis of HEK-293 using 12548-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 12548-1-AP

    IP experiment of HEK-293 using 12548-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.

  • IHC staining of human liver cancer using 12548-1-AP

    IHC staining of human liver cancer using 12548-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 12548-1-AP (CPVL antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 12548-1-AP

    IHC staining of human liver cancer using 12548-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 12548-1-AP (CPVL antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HepG2 using 12548-1-AP

    IF Staining of HepG2 using 12548-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 12548-1-AP (CPVL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 12548-1-AP

    FC experiment of HepG2 using 12548-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CPVL Polyclonal antibody (12548-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, human heart tissue, human kidney tissue, human liver tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

12548-1-AP targets CPVL in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CPVL fusion protein Ag3398 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name carboxypeptidase, vitellogenic-like
Synonyms VCP like protein, UNQ197/PRO223, PSEC0124, hVLP, EC:3.4.16.-
Calculated Molecular Weight 476 aa, 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC016838
Gene Symbol CPVL
Gene ID (NCBI) 54504
RRIDAB_2084865
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H3G5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CPVL antibody 12548-1-APDownload protocol
IHC protocol for CPVL antibody 12548-1-APDownload protocol
IF protocol for CPVL antibody 12548-1-APDownload protocol
IP protocol for CPVL antibody 12548-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Pharmacol

Dissecting gastric cancer heterogeneity and exploring therapeutic strategies using bulk and single-cell transcriptomic analysis and experimental validation of tumor microenvironment and metabolic interplay

Authors - XianTao Lin
View Article