CRABP1 Polyclonal antibody

CRABP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12588-1-AP

Print datasheet

Synonyms

CRABP, CRABP I, CRABP1, CRABPI, RBP5



经过测试的应用

Positive WB detected inmouse embryo tissue, human spleen tissue, Transfected HEK-293 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12588-1-AP targets CRABP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, chicken, rabbit
Immunogen CRABP1 fusion protein Ag3291 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cellular retinoic acid binding protein 1
Synonyms CRABP, CRABP I, CRABP1, CRABPI, RBP5
Calculated Molecular Weight 137 aa, 15 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC022069
Gene Symbol CRABP1
Gene ID (NCBI) 1381
RRIDAB_2292271
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29762
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The cellular retinoic acid-binding proteins including CRABP1 and CRABP2 bind retinoic acid (RA), an important regulator of cell growth and differentiation, thus play an important role in RA-mediated differentiation and proliferation processes. It has been reported that CRABP1 influences the biological effects of RA in cell-selective manners by enhancing the physiological function of RA in keratinocytes or inhibiting RA-induced differentiation of neuroblastoma cells.

实验方案

Product Specific Protocols
WB protocol for CRABP1 antibody 12588-1-APDownload protocol
IHC protocol for CRABP1 antibody 12588-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
chickenIF

Elife

Single-cell proteomics reveals changes in expression during hair-cell development.

Authors - Ying Zhu
humanWB,IHC

Mol Oncol

CRABP-II methylation: a critical determinant of retinoic acid resistance of medulloblastoma cells.

Authors - Fu Yuan-Shan YS
humanWB

Cell Rep

Greater epithelial ridge cells are the principal organoid-forming progenitors of the mouse cochlea.

Authors - Marie Kubota
mouseWB

Virol J

All-trans retinoic acid increases the pathogenicity of the H9N2 influenza virus in mice

Authors - Xiaofei Niu

Cell Rep

Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration

Authors - Lixiang Sun
rabbitWB

Biol Reprod

4D label-free quantitative proteomic analysis identifies CRABP1 as a novel candidate gene for litter size in rabbits

Authors - Zhiyuan Bao