验证数据展示
经过测试的应用
| Positive WB detected in | mouse embryo tissue, human spleen tissue, Transfected HEK-293 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12588-1-AP targets CRABP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, chicken, rabbit |
| 免疫原 | CRABP1 fusion protein Ag3291 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cellular retinoic acid binding protein 1 |
| 别名 | CRABP, CRABP I, CRABP1, CRABPI, RBP5 |
| 计算分子量 | 137 aa, 15 kDa |
| 观测分子量 | 15 kDa |
| GenBank蛋白编号 | BC022069 |
| 基因名称 | CRABP1 |
| Gene ID (NCBI) | 1381 |
| RRID | AB_2292271 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P29762 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The cellular retinoic acid-binding proteins including CRABP1 and CRABP2 bind retinoic acid (RA), an important regulator of cell growth and differentiation, thus play an important role in RA-mediated differentiation and proliferation processes. It has been reported that CRABP1 influences the biological effects of RA in cell-selective manners by enhancing the physiological function of RA in keratinocytes or inhibiting RA-induced differentiation of neuroblastoma cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CRABP1 antibody 12588-1-AP | Download protocol |
| IHC protocol for CRABP1 antibody 12588-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Oncol CRABP-II methylation: a critical determinant of retinoic acid resistance of medulloblastoma cells. | ||
Cell Rep Greater epithelial ridge cells are the principal organoid-forming progenitors of the mouse cochlea. | ||
Virol J All-trans retinoic acid increases the pathogenicity of the H9N2 influenza virus in mice | ||
Cell Rep Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration | ||
Biol Reprod 4D label-free quantitative proteomic analysis identifies CRABP1 as a novel candidate gene for litter size in rabbits |
