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Species Reactivity
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Species Reactivity
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Conjugate
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0
Conjugate
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验证数据展示

  • WB analysis of T-47D using 66468-1-Ig (same clone as 66468-1-PBS)

    WB analysis of T-47D using 66468-1-Ig (same clone as 66468-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 66468-1-Ig (CRABP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66468-1-Ig (same clone as 66468-1-PBS)

    WB analysis of HEK-293 using 66468-1-Ig (same clone as 66468-1-PBS)

    WB result of CRABP2 antibody (66468-1-Ig; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRABP2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

  • IF Staining of human skin cancer using 66468-1-Ig (same clone as 66468-1-PBS)

    IF Staining of human skin cancer using 66468-1-Ig (same clone as 66468-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using CRABP2 antibody (66468-1-Ig, Clone: 1A5F3 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66468-1-Ig (same clone as 66468-1-PBS)

    IHC staining of human ovary tumor using 66468-1-Ig (same clone as 66468-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66468-1-Ig (CRABP2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66468-1-Ig (same clone as 66468-1-PBS)

    IHC staining of human ovary tumor using 66468-1-Ig (same clone as 66468-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66468-1-Ig (CRABP2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 66468-1-Ig (same clone as 66468-1-PBS)

    IF Staining of MCF-7 using 66468-1-Ig (same clone as 66468-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CRABP2 antibody (66468-1-Ig, Clone: 1A5F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 66468-1-Ig (same clone as 66468-1-PBS)

    FC experiment of MCF-7 using 66468-1-Ig (same clone as 66468-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human CRABP2 (66468-1-Ig, Clone:1A5F3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CRABP2 Monoclonal antibody, PBS Only

CRABP2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Cat No. 66468-1-PBS

产品说明书

CloneNo. 1A5F3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

别名

CRABP-II, CRABP II, Cellular retinoic acid-binding protein II, Cellular retinoic acid-binding protein 2, Cellular retinoic acid binding protein II

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66468-1-PBS targets CRABP2 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 CRABP2 fusion protein Ag0309 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cellular retinoic acid binding protein 2
别名 CRABP-II, CRABP II, Cellular retinoic acid-binding protein II, Cellular retinoic acid-binding protein 2, Cellular retinoic acid binding protein II
计算分子量 16 kDa
观测分子量 14 kDa
GenBank蛋白编号BC001109
基因名称 CRABP2
Gene ID (NCBI) 1382
RRIDAB_2881836
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP29373
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.