• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

CRABP2 Monoclonal antibody, PBS Only

CRABP2 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1A5F3

Cat no : 66468-1-PBS

Print datasheet

Synonyms

CRABP-II, CRABP II, Cellular retinoic acid-binding protein II, Cellular retinoic acid-binding protein 2, Cellular retinoic acid binding protein II

验证数据展示

  • WB analysis of T-47D using 66468-1-Ig (same clone as 66468-1-PBS)

    WB analysis of T-47D using 66468-1-Ig (same clone as 66468-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 66468-1-Ig (CRABP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66468-1-Ig (same clone as 66468-1-PBS)

    WB analysis of HEK-293 using 66468-1-Ig (same clone as 66468-1-PBS)

    WB result of CRABP2 antibody (66468-1-Ig; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRABP2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

  • IF Staining of human skin cancer using 66468-1-Ig (same clone as 66468-1-PBS)

    IF Staining of human skin cancer using 66468-1-Ig (same clone as 66468-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using CRABP2 antibody (66468-1-Ig, Clone: 1A5F3 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66468-1-Ig (same clone as 66468-1-PBS)

    IHC staining of human ovary tumor using 66468-1-Ig (same clone as 66468-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66468-1-Ig (CRABP2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66468-1-Ig (same clone as 66468-1-PBS)

    IHC staining of human ovary tumor using 66468-1-Ig (same clone as 66468-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66468-1-Ig (CRABP2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 66468-1-Ig (same clone as 66468-1-PBS)

    FC experiment of MCF-7 using 66468-1-Ig (same clone as 66468-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human CRABP2 (66468-1-Ig, Clone:1A5F3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

产品信息

66468-1-PBS targets CRABP2 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF-P, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen CRABP2 fusion protein Ag0309 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cellular retinoic acid binding protein 2
Synonyms CRABP-II, CRABP II, Cellular retinoic acid-binding protein II, Cellular retinoic acid-binding protein 2, Cellular retinoic acid binding protein II
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC001109
Gene Symbol CRABP2
Gene ID (NCBI) 1382
RRIDAB_2881836
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP29373
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.