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  • Featured Product
  • KD/KO Validated

CRABP2 Monoclonal antibody, PBS Only

CRABP2 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1A5F3

Cat no : 66468-1-PBS

Print datasheet

Synonyms

CRABP-II, CRABP II, Cellular retinoic acid-binding protein II, Cellular retinoic acid-binding protein 2, Cellular retinoic acid binding protein II

验证数据展示

  • WB analysis of T-47D using 66468-1-Ig (same clone as 66468-1-PBS)

    WB analysis of T-47D using 66468-1-Ig (same clone as 66468-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 66468-1-Ig (CRABP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66468-1-Ig (same clone as 66468-1-PBS)

    WB analysis of HEK-293 using 66468-1-Ig (same clone as 66468-1-PBS)

    WB result of CRABP2 antibody (66468-1-Ig; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRABP2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

  • IF Staining of human skin cancer using 66468-1-Ig (same clone as 66468-1-PBS)

    IF Staining of human skin cancer using 66468-1-Ig (same clone as 66468-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using CRABP2 antibody (66468-1-Ig, Clone: 1A5F3 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66468-1-Ig (same clone as 66468-1-PBS)

    IHC staining of human ovary tumor using 66468-1-Ig (same clone as 66468-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66468-1-Ig (CRABP2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66468-1-Ig (same clone as 66468-1-PBS)

    IHC staining of human ovary tumor using 66468-1-Ig (same clone as 66468-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66468-1-Ig (CRABP2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 66468-1-Ig (same clone as 66468-1-PBS)

    FC experiment of MCF-7 using 66468-1-Ig (same clone as 66468-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human CRABP2 (66468-1-Ig, Clone:1A5F3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

产品信息

66468-1-PBS targets CRABP2 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF-P, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen CRABP2 fusion protein Ag0309 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cellular retinoic acid binding protein 2
Synonyms CRABP-II, CRABP II, Cellular retinoic acid-binding protein II, Cellular retinoic acid-binding protein 2, Cellular retinoic acid binding protein II
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC001109
Gene Symbol CRABP2
Gene ID (NCBI) 1382
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP29373
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.