- Featured Product
- KD/KO Validated
CRAT Polyclonal antibody
CRAT Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, mouse heart tissue, rat skeletal muscle, rat heart tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | mouse brain tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:800-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 11 publications below |
产品信息
15170-1-AP targets CRAT in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IP, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, sheep, goat |
| Immunogen | CRAT fusion protein Ag7422 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | carnitine acetyltransferase |
| Synonyms | EC:2.3.1.137, CAT1, CAT, Carnitine O-acetyltransferase, Carnitine acetylase |
| Calculated Molecular Weight | 71 kDa |
| Observed Molecular Weight | 62-68 kDa |
| GenBank Accession Number | BC000723 |
| Gene Symbol | CRAT |
| Gene ID (NCBI) | 1384 |
| RRID | AB_2229978 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P43155 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CRAT, also named as CAT1, belongs to the carnitine/choline acetyltransferase family. It is specific for short chain fatty acids. CRAT seems to affect the flux through the pyruvate dehydrogenase complex. It may be involved as well in the transport of acetyl-CoA into mitochondria. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CRAT is an active forms for carnitine acetyltransferase. This antibody can bind the close sequences genes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CRAT antibody 15170-1-AP | Download protocol |
| IHC protocol for CRAT antibody 15170-1-AP | Download protocol |
| IP protocol for CRAT antibody 15170-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Dissociation of Muscle Insulin Resistance from Alterations in Mitochondrial Substrate Preference. | ||
Cancer Res Organelle-Derived Acetyl-CoA Promotes Prostate Cancer Cell Survival, Migration, and Metastasis via Activation of Calmodulin Kinase II. | ||
Redox Biol TGF-β1 attenuates mitochondrial bioenergetics in pulmonary arterial endothelial cells via the disruption of carnitine homeostasis.
| ||
Antioxid Redox Signal Disruption of endothelial cell mitochondrial bioenergetics in lambs with increased pulmonary blood flow. | ||
Mol Metab Carnitine acetyltransferase (CRAT) expression in macrophages is dispensable for nutrient stress sensing and inflammation. | ||
Heliyon Stable isotope-labeled carnitine reveals its rapid transport into muscle cells and acetylation during contraction |
