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  • Featured Product
  • KD/KO Validated

CRBN Polyclonal antibody

CRBN Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 28494-1-AP

Print datasheet

Synonyms

Protein cereblon, cereblon, AD-006

验证数据展示

  • WB analysis using 28494-1-AP

    WB analysis using 28494-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28494-1-AP (CRBN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 28494-1-AP

    WB analysis using 28494-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28494-1-AP (CRBN antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 28494-1-AP

    IHC staining of human liver cancer using 28494-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28494-1-AP (CRBN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 28494-1-AP

    IHC staining of human liver cancer using 28494-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28494-1-AP (CRBN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 28494-1-AP

    IHC staining of mouse brain using 28494-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28494-1-AP (CRBN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 28494-1-AP

    IHC staining of mouse brain using 28494-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28494-1-AP (CRBN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of COLO 320 using 28494-1-AP

    IF Staining of COLO 320 using 28494-1-AP

    Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using CRBN antibody (28494-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inCOLO 320 cells, mouse liver tissue, 3T3-L1 cells, THP-1 cells, U-251 cells, C6 cells
Positive IHC detected inmouse brain tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCOLO 320 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28494-1-AP targets CRBN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CRBN fusion protein Ag29353 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cereblon
Synonyms Protein cereblon, cereblon, AD-006
Calculated Molecular Weight 442 aa, 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC017419
Gene Symbol CRBN
Gene ID (NCBI) 51185
RRIDAB_2881155
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96SW2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRBN plays an important role in assembly and surface expression of functional BK(Ca) channels. The mutation of human cereblon gene (CRBN) is related with mild mental retardation, and causes ARNSID (nonsyndromal intellectual disability) by distubing the developmental regulation of BK(Ca) in brain regions that are critical for memory and learning. CRBN is also identified as important thalidomide-binding protein for limb outgrowth in zebrafish and chicks. It can be detected a band above 51 kDa (maybe 55 kDa) in HT22 cells(PMID: 21232561).

实验方案

Product Specific Protocols
WB protocol for CRBN antibody 28494-1-APDownload protocol
IHC protocol for CRBN antibody 28494-1-APDownload protocol
IF protocol for CRBN antibody 28494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Leukemia

Phase 2 study of oral thalidomide-cyclophosphamide-dexamethasone for recurrent/refractory adult Langerhans cell histiocytosis.

Authors - Ji-Nuo Wang
View Article
humanWB,IF,IP

Hum Mol Genet

Cereblon suppresses the formation of pathogenic protein aggregates in a p62-dependent manner.

Authors - Liang Zhou
  • KD Validated
View Article
WB

J Med Chem

Discovery of Novel Proteolysis-Targeting Chimera Molecules as Degraders of Programmed Cell Death-Ligand 1 for Breast Cancer Therapy

Authors - Hongjia Zhang
  • KD Validated
View Article