验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, mouse liver tissue, 3T3-L1 cells, THP-1 cells, U-251 cells, C6 cells |
| Positive IHC detected in | mouse brain tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | COLO 320 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28494-1-AP targets CRBN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | CRBN fusion protein Ag29353 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cereblon |
| 别名 | Protein cereblon, cereblon, AD-006 |
| 计算分子量 | 442 aa, 51 kDa |
| 观测分子量 | 51 kDa |
| GenBank蛋白编号 | BC017419 |
| 基因名称 | CRBN |
| Gene ID (NCBI) | 51185 |
| RRID | AB_2881155 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96SW2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CRBN plays an important role in assembly and surface expression of functional BK(Ca) channels. The mutation of human cereblon gene (CRBN) is related with mild mental retardation, and causes ARNSID (nonsyndromal intellectual disability) by distubing the developmental regulation of BK(Ca) in brain regions that are critical for memory and learning. CRBN is also identified as important thalidomide-binding protein for limb outgrowth in zebrafish and chicks. It can be detected a band above 51 kDa (maybe 55 kDa) in HT22 cells(PMID: 21232561).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CRBN antibody 28494-1-AP | Download protocol |
| IHC protocol for CRBN antibody 28494-1-AP | Download protocol |
| IF protocol for CRBN antibody 28494-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Leukemia Phase 2 study of oral thalidomide-cyclophosphamide-dexamethasone for recurrent/refractory adult Langerhans cell histiocytosis. | ||
Hum Mol Genet Cereblon suppresses the formation of pathogenic protein aggregates in a p62-dependent manner.
| ||
J Med Chem Discovery of Novel Proteolysis-Targeting Chimera Molecules as Degraders of Programmed Cell Death-Ligand 1 for Breast Cancer Therapy
| ||
Cell Death Discov Arginine methylation-dependent TRIM47 stability mediated by CARM1 promotes the metastasis of hepatocellular carcinoma | ||
Acta Pharm Sin B Discovery of a potent PROTAC degrader for RNA demethylase FTO as antileukemic therapy |
