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- KD/KO Validated
CREB3 Polyclonal antibody
CREB3 Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IF/ICC, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells |
| Positive IF detected in | U2OS cells |
Planning an IHC experiment? We recommend our IHCeasy CREB3 Ready-To-Use IHC Kit. CREB3 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11275-1-AP targets CREB3 in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IP, IF, IHC, ChIP |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | CREB3 fusion protein Ag1823 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cAMP responsive element binding protein 3 |
| Synonyms | CREB 3, CREB3, LUMAN, LZIP |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 40-64 kDa |
| GenBank Accession Number | BC009402 |
| Gene Symbol | CREB3 |
| Gene ID (NCBI) | 10488 |
| RRID | AB_10640533 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43889 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CREB3, also named as LZIP and Luman, belongs to the bZIP family and ATF subfamily. It is a transcription factors activated upon intramembrane proteolysis (RIP), binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. CREB3 binds to and requires HCFC1 as a coactivator. The activity and expression are suppressed when the HCFC1-CREB3 complex binds with CREBZF. Participates in LKN-1/CCL15-induced chemotaxis signaling. CREB3 is N-glycosylated and released by proteolysis. So the MW of CREB3 emirate 40kd to 64kd (PMID: 12138176).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CREB3 antibody 11275-1-AP | Download protocol |
| IHC protocol for CREB3 antibody 11275-1-AP | Download protocol |
| IF protocol for CREB3 antibody 11275-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Standalone or combinatorial phenylbutyrate therapy shows excellent antiviral activity and mimics CREB3 silencing.
| ||
Nat Commun Golgi stress induces SIRT2 to counteract Shigella infection via defatty-acylation. | ||
Int J Mol Sci Comparative Analysis of CREB3 and CREB3L2 Protein Expression in HEK293 Cells. | ||
Cancers (Basel) miR766-3p and miR124-3p Dictate Drug Resistance and Clinical Outcome in HNSCC | ||
Front Mol Neurosci LUMAN/CREB3 Plays a Dual Role in Stress Responses as a Cofactor of the Glucocorticoid Receptor and a Regulator of Secretion. | ||
Carcinogenesis Small leucine zipper protein promotes the metastasis of castration-resistant prostate cancer through transcriptional regulation of matrix metalloproteinase-13. |
