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CRELD2 Polyclonal antibody

CRELD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27017-1-AP

Print datasheet

Synonyms

CRELD2

验证数据展示

  • WB analysis of human placenta using 27017-1-AP

    WB analysis of human placenta using 27017-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 27017-1-AP (CRELD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 27017-1-AP

    IHC staining of human placenta using 27017-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27017-1-AP (CRELD2 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human placenta using 27017-1-AP

    IHC staining of human placenta using 27017-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27017-1-AP (CRELD2 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inhuman placenta tissue, SH-SY5Y cells, U2OS cells
Positive IHC detected inhuman placenta tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27017-1-AP targets CRELD2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen CRELD2 fusion protein Ag25553 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cysteine-rich with EGF-like domains 2
Synonyms CRELD2
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 30-45 kDa
GenBank Accession NumberBC050675
Gene Symbol CRELD2
Gene ID (NCBI) 79174
RRIDAB_2880723
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UXH1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CRELD2 antibody 27017-1-APDownload protocol
IHC protocol for CRELD2 antibody 27017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Ther

Ectopic clotting factor VIII expression and misfolding in hepatocytes as a cause for hepatocellular carcinoma

Authors - Audrey Kapelanski-Lamoureux
View Article