CREST Monoclonal antibody
CREST Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, rat, mouse
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
3D5D10
验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, COLO 320 cells, HSC-T6 cells, RAW 264.7 cells, MCF-7 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60314-1-Ig targets CREST in WB, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | human, rat, mouse |
| Immunogen | CREST fusion protein Ag3119 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | synovial sarcoma translocation gene on chromosome 18-like 1 |
| Synonyms | CREST, KIAA0693, LP2261, SS18 like protein 1, SS18L1, SYT homolog 1 |
| Calculated Molecular Weight | 396 aa, 43 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC034494 |
| Gene Symbol | CREST |
| Gene ID (NCBI) | 26039 |
| RRID | AB_2881425 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O75177 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SS18-like 1(SS18L1) is a transcriptional activator that is required for calcium-dependent dendritic growth and branching in cortical neurons. It's also a nuclear protein interacts with CREB-binding protein and expressed in the developing brain. It helps regulate neuronal morphogenesis in calcuim-dependent manner. The N-terminal domain of SS18L1 is required for suppressing transactivation in the basal state, while the C-terminal domain is required for calcium-induced transactivation. It's part of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. This complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. The calculated molecular weight of CREST is about 43 kDa, but the modified of CREST protein is 55 kDa (PMID: 25888396).
