验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, COLO 320 cells, HSC-T6 cells, RAW 264.7 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60314-1-Ig targets CREST in WB, ELISA applications and shows reactivity with human, rat, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, rat, mouse |
| 免疫原 | CREST fusion protein Ag3119 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | synovial sarcoma translocation gene on chromosome 18-like 1 |
| 别名 | CREST, KIAA0693, LP2261, SS18 like protein 1, SS18L1, SYT homolog 1 |
| 计算分子量 | 396 aa, 43 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC034494 |
| 基因名称 | CREST |
| Gene ID (NCBI) | 26039 |
| RRID | AB_2881425 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O75177 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SS18-like 1(SS18L1) is a transcriptional activator that is required for calcium-dependent dendritic growth and branching in cortical neurons. It's also a nuclear protein interacts with CREB-binding protein and expressed in the developing brain. It helps regulate neuronal morphogenesis in calcuim-dependent manner. The N-terminal domain of SS18L1 is required for suppressing transactivation in the basal state, while the C-terminal domain is required for calcium-induced transactivation. It's part of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. This complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. The calculated molecular weight of CREST is about 43 kDa, but the modified of CREST protein is 55 kDa (PMID: 25888396).
