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CREST Monoclonal antibody

CREST Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

3D5D10

Cat No : 60314-1-Ig

Print datasheet

Synonyms

CREST, KIAA0693, LP2261, SS18 like protein 1, SS18L1, SYT homolog 1

验证数据展示

  • WB analysis of NIH/3T3 using 60314-1-Ig

    WB analysis of NIH/3T3 using 60314-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

产品信息

60314-1-Ig targets CREST in WB, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications WB,ELISA Application Description
Tested Reactivity human, rat, mouse
Immunogen CREST fusion protein Ag3119 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name synovial sarcoma translocation gene on chromosome 18-like 1
Synonyms CREST, KIAA0693, LP2261, SS18 like protein 1, SS18L1, SYT homolog 1
Calculated Molecular Weight 396 aa, 43 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC034494
Gene Symbol SS18L1
Gene ID (NCBI) 26039
RRIDAB_2881425
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75177
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SS18-like 1(SS18L1) is a transcriptional activator that is required for calcium-dependent dendritic growth and branching in cortical neurons. It's also a nuclear protein interacts with CREB-binding protein and expressed in the developing brain. It helps regulate neuronal morphogenesis in calcuim-dependent manner. The N-terminal domain of SS18L1 is required for suppressing transactivation in the basal state, while the C-terminal domain is required for calcium-induced transactivation. It's part of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. This complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. The calculated molecular weight of CREST is about 43 kDa, but the modified of CREST protein is 55 kDa (PMID: 25888396).