验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, rat brain tissue |
| Positive IHC detected in | human pancreas cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10944-1-AP targets CRH/CRF in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, zebrafish, brandt’s voles, microtus ochrogaster |
| 免疫原 | CRH/CRF fusion protein Ag1371 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CRH/CRF |
| 别名 | Corticoliberin, CRF, CRH |
| 计算分子量 | 21 kDa |
| 观测分子量 | 35-38 kDa |
| GenBank蛋白编号 | BC011031 |
| 基因名称 | CRH |
| Gene ID (NCBI) | 1392 |
| RRID | AB_2084279 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P06850 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CRH, also called CRF or corticoliberin, is a peptide hormone and neurotransmitter involved in the stress response. Marked reduction in this protein has been observed in association with Alzheimer disease. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CRH/CRF antibody 10944-1-AP | Download protocol |
| IHC protocol for CRH/CRF antibody 10944-1-AP | Download protocol |
| IF protocol for CRH/CRF antibody 10944-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neurobiol Stress Social isolation alters behavior, the gut-immune-brain axis, and neurochemical circuits in male and female prairie voles. | ||
Elife Single-cell transcriptome analysis identifies a unique tumor cell type producing multiple hormones in ectopic ACTH and CRH secreting pheochromocytoma. | ||
Brain Behav Immun Defects in NLRP6, autophagy and goblet cell homeostasis are associated with reduced duodenal CRH receptor 2 expression in patients with functional dyspepsia. | ||
Int J Mol Sci Stress and Nasal Allergy: Corticotropin-Releasing Hormone Stimulates Mast Cell Degranulation and Proliferation in Human Nasal Mucosa. | ||
Development PLXNA1 and PLXNA3 cooperate to pattern the nasal axons that guide gonadotropin-releasing hormone neurons. | ||
J Ethnopharmacol Antidepressant and Anxiolytic Effects of Wuling Capsule in CSDS Mice: Alleviating HPA Axis Hyperactivity via the Nesfatin-1/NF-κB Signaling Pathway |
