验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, SH-SY5Y cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:200-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20967-1-AP targets CRHR1 in WB, IF, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | corticotropin releasing hormone receptor 1 |
别名 | CRF R, CRF R 1, CRF R1, CRF1, CRF1R, CRFR, CRFR 1, CRFR1, CRH R 1, CRH R1, CRH R1h, CRHR, CRHR1, CRHR1f |
计算分子量 | 48 kDa |
观测分子量 | 46-55 kDa |
GenBank蛋白编号 | NM_004382 |
基因名称 | CRHR1 |
Gene ID (NCBI) | 1394 |
RRID | AB_2878776 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P34998 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Corticotropin-releasing hormone (CRH, also known as Corticotropin-releasing factor, CRF) is a 41 amino acid peptide hormone and a key regulator of the stress response. CRH and the related urocortin peptides (UCN, UCN2 and UCN3) mediate their actions through two mammalian receptor subtypes, CRHR1 and CRHR2 (PMID: 23863939). CRHR1 (also known as CRFR1) exhibits high affinity towards CRH and UCN but low affinity towards UCN2 and no affinity towards UCN3 (PMID: 22684850). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Widely distributed throughout the brain, CRHR1 critically controls behavioral adaptation to stress and is causally linked to emotional disorders (PMID: 21885734). Alternative splicing results in multiple transcript variants encoding distinct isoforms of CRHR1. This antibody raised against a synthesized peptide corresponding to amino acid residues 148-165 of isoform CRF-R1 can only recognize isoform CRF-R1.
实验方案
Product Specific Protocols | |
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WB protocol for CRHR1 antibody 20967-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Proc Natl Acad Sci U S A Overactivation of corticotropin-releasing factor receptor type 1 and aquaporin-4 by hypoxia induces cerebral edema. | ||
Pharmaceuticals (Basel) A Novel Mast Cell Stabilizer JM25-1 Rehabilitates Impaired Gut Barrier by Targeting the Corticotropin-Releasing Hormone Receptors | ||
Behav Brain Res Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats. | ||
Brain Res Response of the maternal hypothalamus to cold stress during late pregnancy in rats. | ||
Int J Mol Med Sini San ameliorates duodenal mucosal barrier injury and low‑grade inflammation via the CRF pathway in a rat model of functional dyspepsia. | ||
Reprod Toxicol Dynamic expression of corticotropin-releasing hormone and urocortin in estrogen induced-cholestasis pregnant rat. |