验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, PC-3 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15349-1-AP targets CRIP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | CRIP1 fusion protein Ag7588 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cysteine-rich protein 1 (intestinal) |
| 别名 | CRP-1, CRP1, CRP 1, CRIP 1, CRIP |
| 计算分子量 | 9 kDa |
| 观测分子量 | 8-9 kDa |
| GenBank蛋白编号 | BC002738 |
| 基因名称 | CRIP1 |
| Gene ID (NCBI) | 1396 |
| RRID | AB_2878128 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P50238 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CRIP1 also known as CRIP, CRP1, belongs to the LIM/double zinc finger protein family. CRIP1 has a unique double zinc finger motif, which is mainly expressed in the intestine, and may be involved in intestinal zinc transport (PMID: 31312368, 1946385). CRIP1 is overexpressed in immune cells of the epithelium and may play an important role in gut immunity(PMID: 27836662).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CRIP1 antibody 15349-1-AP | Download protocol |
| IHC protocol for CRIP1 antibody 15349-1-AP | Download protocol |
| IF protocol for CRIP1 antibody 15349-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EBioMedicine CRIP1 involves the pathogenesis of multiple myeloma via dual-regulation of proteasome and autophagy
| ||
Dis Markers Cysteine-Rich Intestinal Protein 1 Served as an Epithelial Ovarian Cancer Marker via Promoting Wnt/β-Catenin-Mediated EMT and Tumour Metastasis.
| ||
Leuk Res CRIP1 supports the growth and migration of AML-M5 subtype cells by activating Wnt/β-catenin pathway
| ||
EMBO J CRIP1 suppresses BBOX1-mediated carnitine metabolism to promote stemness in hepatocellular carcinoma |
