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  • KD/KO Validated

CRIP1 Polyclonal antibody

CRIP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 15349-1-AP

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Synonyms

CRP-1, CRP1, CRP 1, CRIP 1, CRIP



经过测试的应用

Positive WB detected inHeLa cells, PC-3 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15349-1-AP targets CRIP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IP, CoIP
Tested Reactivity human
Cited Reactivityhuman
Immunogen CRIP1 fusion protein Ag7588 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cysteine-rich protein 1 (intestinal)
Synonyms CRP-1, CRP1, CRP 1, CRIP 1, CRIP
Calculated Molecular Weight 9 kDa
Observed Molecular Weight 8-9 kDa
GenBank Accession NumberBC002738
Gene Symbol CRIP1
Gene ID (NCBI) 1396
RRIDAB_2878128
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP50238
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRIP1 also known as CRIP, CRP1, belongs to the LIM/double zinc finger protein family. CRIP1 has a unique double zinc finger motif, which is mainly expressed in the intestine, and may be involved in intestinal zinc transport (PMID: 31312368, 1946385). CRIP1 is overexpressed in immune cells of the epithelium and may play an important role in gut immunity(PMID: 27836662).

实验方案

Product Specific Protocols
WB protocol for CRIP1 antibody 15349-1-APDownload protocol
IHC protocol for CRIP1 antibody 15349-1-APDownload protocol
IF protocol for CRIP1 antibody 15349-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,CoIP

EBioMedicine

CRIP1 involves the pathogenesis of multiple myeloma via dual-regulation of proteasome and autophagy

Authors - Peixia Tang
  • KD Validated
humanWB

Dis Markers

Cysteine-Rich Intestinal Protein 1 Served as an Epithelial Ovarian Cancer Marker via Promoting Wnt/β-Catenin-Mediated EMT and Tumour Metastasis.

Authors - Yujuan Liu
  • KD Validated
humanWB,CoIP

Leuk Res

CRIP1 supports the growth and migration of AML-M5 subtype cells by activating Wnt/β-catenin pathway

Authors - Xiaoling Deng
  • KD Validated
humanIHC,IP

EMBO J

CRIP1 suppresses BBOX1-mediated carnitine metabolism to promote stemness in hepatocellular carcinoma

Authors - Jing Wang