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验证数据展示

  • WB analysis using 83340-3-RR

    WB analysis using 83340-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83340-3-RR (CRIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 83340-3-RR

    IHC staining of human colon using 83340-3-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 83340-3-RR (CRIP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83340-3-RR

    Affinity and Kinetic Characterization of 83340-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83340-3-RR against Human CRIP1 were performed. The affinity constant is 0.784 nM.

CRIP1 Recombinant antibody

CRIP1 Recombinant Antibody for WB, IHC, ELISA
Cat No. 83340-3-RR

产品说明书

CloneNo. 240176G2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

CRP1, CRP 1, CRIP 1, CRIP, CRHP

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inT-47D cells, HeLa cells, mouse colon tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83340-3-RR targets CRIP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 CRIP1 fusion protein Ag7588 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cysteine-rich protein 1 (intestinal)
别名 CRP1, CRP 1, CRIP 1, CRIP, CRHP
计算分子量 9 kDa
观测分子量 8-9 kDa
GenBank蛋白编号BC002738
基因名称 CRIP1
Gene ID (NCBI) 1396
RRIDAB_3671000
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP50238
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRIP1 also known as CRIP, CRP1, belongs to the LIM/double zinc finger protein family. CRIP1 has a unique double zinc finger motif, which is mainly expressed in the intestine, and may be involved in intestinal zinc transport (PMID: 31312368, 1946385). CRIP1 is overexpressed in immune cells of the epithelium and may play an important role in gut immunity(PMID: 27836662).

实验方案

Product Specific Protocols
WB protocol for CRIP1 antibody 83340-3-RRDownload protocol
IHC protocol for CRIP1 antibody 83340-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols