验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, K-562 cells, Jurkat cells |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26816-1-AP targets CDK12/CRKRS in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | CDK12/CRKRS fusion protein Ag25323 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Cdc2-related kinase, arginine/serine-rich |
| 别名 | CrkRS, CRK7, Cell division cycle 2-related protein kinase 7, CDK12, CDC2-related protein kinase 7 |
| 计算分子量 | 1490 aa, 164 kDa |
| 观测分子量 | 180-200 kDa |
| GenBank蛋白编号 | BC150265 |
| 基因名称 | CDK12 |
| Gene ID (NCBI) | 51755 |
| RRID | AB_2880645 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NYV4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CRKRS (Cdc2-related kinase, Arg/Ser), also named as cyclin-dependent kinase 12 (CKD12), belongs to CDC2/CDKX subfamily. CDK12 can form a heterodimeric complex with its activating partner, cyclin K, to regulate important cellular physiological processes that include transcriptional regulation, regulation of DNA damage repair genes such as BRCA1 and ATM, suppression of intronic polyadenylation, and susceptibility to PARP inhibitors (PMID: 30487607, 24240700, 31694772). CDK12 has 3 isoforms with the calculated molecular mass of 164/163/141 kDa, and the apparent molecular mass of 180-200 kDa due to some modifications (PMID 11683387, 22988298).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CDK12/CRKRS antibody 26816-1-AP | Download protocol |
| IF protocol for CDK12/CRKRS antibody 26816-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep Med Development of an orally bioavailable CDK12/13 degrader and induction of synthetic lethality with AKT pathway inhibition | ||
Eur J Cancer CDK12 inhibition enhances sensitivity of HER2+ breast cancers to HER2-tyrosine kinase inhibitor via suppressing PI3K/AKT. | ||
iScience THOC5 complexes with DDX5, DDX17, and CDK12 to regulate R loop structures and transcription elongation rate | ||
Int J Med Sci Expression pattern of CDK12 protein in gastric cancer and its positive correlation with CD8+ cell density and CCL12 expression. | ||
Cell Death Discov JWA suppresses proliferation in trastuzumab-resistant breast cancer by downregulating CDK12.
| ||
Nucleic Acids Res Identifying human pre-mRNA cleavage and polyadenylation factors by genome-wide CRISPR screens using a dual fluorescence readthrough reporter |
