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验证数据展示

  • WB analysis using 11567-1-AP

    WB analysis using 11567-1-AP

    U-251 cells were subjected to SDS PAGE followed by western blot with 11567-1-AP (CRLF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CRLF3 Polyclonal antibody

CRLF3 Polyclonal Antibody for WB, ELISA
Cat No. 11567-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

CREME 9, CREME9, CRLF3, CYTOR4, FRWS, P48

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11567-1-AP targets CRLF3 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 CRLF3 fusion protein Ag2144 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytokine receptor-like factor 3
别名 CREME 9, CREME9, CRLF3, CYTOR4, FRWS, P48
计算分子量 442 aa, 50 kDa
观测分子量51 kDa
GenBank蛋白编号BC023567
基因名称 CRLF3
Gene ID (NCBI) 51379
RRIDAB_3085377
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IUI8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytokine receptor-like factor 3 (CRLF3), a member of the group 1 in the prototypic class I cytokine receptors, contains the conserved cytokine receptor motif WSXWS, a single transmembrane segment and an intracellular Janus kinase (JAK) docking site (PMID: 31680856). CRLF3 is expressed in most normal human tissues and many tumor cell lines and has been reported to negatively regulate cell cycle progression (PMID: 19427400). CRLF3 has been identified as a human neuroprotective EV-3 (Epo) receptor (PMID: 37168680). It is involved neurogenesis, neuron survival, and dendritic development (PMID: 34233200).

实验方案

Product Specific Protocols
WB protocol for CRLF3 antibody 11567-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols