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CRMP2 Polyclonal antibody, PBS Only

CRMP2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14686-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 14686-1-AP (same clone as 14686-1-PBS)

    WB analysis using 14686-1-AP (same clone as 14686-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14686-1-AP (CRMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14686-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 14686-1-AP (same clone as 14686-1-PBS)

    IHC staining of mouse brain using 14686-1-AP (same clone as 14686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14686-1-AP (CRMP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14686-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DPYSL2 antibody included
  • IHC staining of mouse brain using 14686-1-AP (same clone as 14686-1-PBS)

    IHC staining of mouse brain using 14686-1-AP (same clone as 14686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14686-1-AP (CRMP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14686-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DPYSL2 antibody included
  • IF Staining of HepG2 using 14686-1-AP (same clone as 14686-1-PBS)

    IF Staining of HepG2 using 14686-1-AP (same clone as 14686-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CRMP2 antibody (14686-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 14686-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy DPYSL2 Ready-To-Use IHC Kit. DPYSL2 primary antibody included.

产品信息

14686-1-PBS targets CRMP2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CRMP2 fusion protein Ag5985 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dihydropyrimidinase-like 2
Synonyms
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 62-66 kDa
GenBank Accession NumberBC056408
Gene Symbol CRMP2
Gene ID (NCBI) 1808
RRIDAB_10638925
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16555
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Dihydropyrimidinase-like 2 (Dpysl2), a protein involved in neuronal differentiation and axonal guidance is found altered in various nervous system diseases (PMID:19524114). It is also named as CRMP2, ULIP2 and belongs to hydantoinase/dihydropyrimidinase subfamily. The phosphorylation of the particular portion carboxyl-terminal to the basic region of DPYSL2 may play an important role in regulating its activity, and may be involved in the formation of degenerating neurites in AD brain(PMID:10757975).