验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HepG2 cells, mouse brain tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human ovary cancer tissue, human ovary tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14504-1-AP targets CROP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | CROP fusion protein Ag5961 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cisplatin resistance-associated overexpressed protein |
| 别名 | CREAP-1, CREAP1, CREAP 1, CRE associated protein 1, Cisplatin resistance-associated-overexpressed protein |
| 计算分子量 | 51 kDa |
| 观测分子量 | 55-58 kDa |
| GenBank蛋白编号 | BC056409 |
| 基因名称 | CROP |
| Gene ID (NCBI) | 51747 |
| RRID | AB_2229967 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95232 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CROP, a putative SR protein, contain cystine/histidine motifs and leucine zipper-like repeats in the N-terminal half and consists mostly of charged and polar amino acids in the C-terminal half. CROP has a high expression in heart, brain, pancreas, thymus, and weak in lung, live and kidney. As the human homologue of yeast Luc7p, CROP is supported to be participated in 5'-splice site recognize and is essential for vegetative growth. This is a rabbit polyclonal antibody raised against part of N-terminus of CROP of human origin, and can recognize a ~58kDa band of CROP.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CROP antibody 14504-1-AP | Download protocol |
| IHC protocol for CROP antibody 14504-1-AP | Download protocol |
| IF protocol for CROP antibody 14504-1-AP | Download protocol |
| IP protocol for CROP antibody 14504-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS Genet Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.
| ||
Cell Rep Functional analyses of human LUC7-like proteins involved in splicing regulation and myeloid neoplasms.
| ||
Curr Issues Mol Biol Proteomics Identifies LUC7L3 as a Prognostic Biomarker for Hepatocellular Carcinoma | ||
Inorg Chem Studies of Anticancer Activities In Vitro and In Vivo for Butyltin(IV)-Iridium(III) Imidazole-Phenanthroline Complexes with Aggregation-Induced Emission Properties | ||
J Biol Chem The NEDD4-binding protein N4BP1 degrades mRNA substrates through the coding sequence independent of nonsense-mediated decay |
