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CROT Polyclonal antibody
CROT Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13543-1-AP targets CROT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | CROT fusion protein Ag4486 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | carnitine O-octanoyltransferase |
| Synonyms | Peroxisomal carnitine O-octanoyltransferase, EC:2.3.1.137, COT |
| Calculated Molecular Weight | 612 aa, 66 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC039004 |
| Gene Symbol | CROT |
| Gene ID (NCBI) | 54677 |
| RRID | AB_2085513 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UKG9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CROT, also named as COT, belongs to the carnitine/choline acetyltransferase family. It is a beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate. CROT converts the end product of pristanic acid beta oxidation, 4,8-dimethylnonanoyl-CoA, to its corresponding carnitine ester. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CROT is an active forms for carnitine octanoyltransferase. This antibody can bind the close sequences genes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CROT antibody 13543-1-AP | Download protocol |
| IHC protocol for CROT antibody 13543-1-AP | Download protocol |
| IF protocol for CROT antibody 13543-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Endocrinol (Lausanne) The miR-33a-5p/CROT axis mediates ovarian cancer cell behaviors and chemoresistance via the regulation of the TGF-β signal pathway
| ||
J Biol Chem Carnitine octanoyltransferase is important for the assimilation of exogenous acetyl-L-carnitine into acetyl-CoA in mammalian cells
| ||
J Biol Chem Carnitine o-octanoyltransferase is a p53 target that promotes oxidative metabolism and cell survival following nutrient starvation
| ||
Am J Transl Res Artemether regulates liver glycogen and lipid utilization through mitochondrial pyruvate oxidation in db/db mice | ||