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CRTAC1 Polyclonal antibody

CRTAC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13001-1-AP

Print datasheet

Synonyms

ASPIC, ASPIC1, cartilage acidic protein 1, CEP 68, CEP68, CRTAC1

验证数据展示

  • WB analysis of human brain using 13001-1-AP

    WB analysis of human brain using 13001-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13001-1-AP (CRTAC1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 13001-1-AP

    IHC staining of mouse lung using 13001-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13001-1-AP (CRTAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 13001-1-AP

    IHC staining of mouse lung using 13001-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13001-1-AP (CRTAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inmouse lung tissue, mouse brain tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13001-1-AP targets CRTAC1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CRTAC1 fusion protein Ag3673 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cartilage acidic protein 1
Synonyms ASPIC, ASPIC1, cartilage acidic protein 1, CEP 68, CEP68, CRTAC1
Calculated Molecular Weight 661 aa, 71 kDa
Observed Molecular Weight 68-72 kDa
GenBank Accession NumberBC034245
Gene Symbol CRTAC1
Gene ID (NCBI) 55118
RRIDAB_10638787
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQ79
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CRTAC1 antibody 13001-1-APDownload protocol
IHC protocol for CRTAC1 antibody 13001-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell Rep

Integrative single-cell meta-analysis reveals disease-relevant vascular cell states and markers in human atherosclerosis

Authors - Jose Verdezoto Mosquera
View Article
humanWB,IHC

Sci Rep

CRTAC1 identified as a promising diagnosis and prognostic biomarker in lung adenocarcinoma

Authors - Lin Tan
View Article
humanWB

Am J Rhinol Allergy

Analysis of CRSsNP Proteome Using a Highly Multiplexed Approach in Nasal Mucus

Authors - Vanessa-Vivien Pesold
View Article