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CRTAC1 Polyclonal antibody

CRTAC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30251-1-AP

Print datasheet

Synonyms

ASPIC, ASPIC1, cartilage acidic protein 1, CEP 68, CEP68, CRTAC1, FLJ10320

验证数据展示

  • WB analysis using 30251-1-AP

    WB analysis using 30251-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30251-1-AP (CRTAC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 30251-1-AP

    WB analysis using 30251-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30251-1-AP (CRTAC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 30251-1-AP

    IHC staining of mouse brain using 30251-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30251-1-AP (CRTAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 30251-1-AP

    IHC staining of rat brain using 30251-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30251-1-AP (CRTAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman plasma, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30251-1-AP targets CRTAC1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen CRTAC1 fusion protein Ag32963 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cartilage acidic protein 1
Synonyms ASPIC, ASPIC1, cartilage acidic protein 1, CEP 68, CEP68, CRTAC1, FLJ10320
Calculated Molecular Weight 661 aa, 71 kDa
Observed Molecular Weight 71-100 kDa
GenBank Accession NumberBC034245
Gene Symbol CRTAC1
Gene ID (NCBI) 55118
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQ79
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRTAC1 (Cartilage acidic protein 1) is also named as ASPIC and 68 kDa chondrocyte-expressed protein (CEP-68). Cartilage acidic protein 1 (CRTAC1), a novel human marker which allowed discrimination of human chondrocytes from osteoblasts and mesenchymal stem cells (PMID: 17074475). CRTAC1 is a glycosylated calcium-binding extracellular matrix protein (PMID: 37796703). CRTAC1 acquired an alternate last exon from the tail-to-tail oriented neighbouring gene in humans resulting in the glycosylated isoform CRTAC1-A which represents a new extracellular matrix molecule of articular cartilage (PMID: 17074475). CRTAC1 was downregulated in bladder cancer tissues and cells (PMID: 34818994).

实验方案

Product Specific Protocols
WB protocol for CRTAC1 antibody 30251-1-APDownload protocol
IHC protocol for CRTAC1 antibody 30251-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols