• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 30251-1-AP

    WB analysis using 30251-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30251-1-AP (CRTAC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 30251-1-AP

    WB analysis using 30251-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30251-1-AP (CRTAC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 30251-1-AP

    IHC staining of mouse brain using 30251-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30251-1-AP (CRTAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 30251-1-AP

    IHC staining of rat brain using 30251-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30251-1-AP (CRTAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CRTAC1 Polyclonal antibody

CRTAC1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30251-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

ASPIC, ASPIC1, cartilage acidic protein 1, CEP 68, CEP68, CRTAC1, FLJ10320

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inhuman plasma, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30251-1-AP targets CRTAC1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 CRTAC1 fusion protein Ag32963 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cartilage acidic protein 1
别名 ASPIC, ASPIC1, cartilage acidic protein 1, CEP 68, CEP68, CRTAC1, FLJ10320
计算分子量 661 aa, 71 kDa
观测分子量 71-100 kDa
GenBank蛋白编号BC034245
基因名称 CRTAC1
Gene ID (NCBI) 55118
RRIDAB_3086281
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQ79
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRTAC1 (Cartilage acidic protein 1) is also named as ASPIC and 68 kDa chondrocyte-expressed protein (CEP-68). Cartilage acidic protein 1 (CRTAC1), a novel human marker which allowed discrimination of human chondrocytes from osteoblasts and mesenchymal stem cells (PMID: 17074475). CRTAC1 is a glycosylated calcium-binding extracellular matrix protein (PMID: 37796703). CRTAC1 acquired an alternate last exon from the tail-to-tail oriented neighbouring gene in humans resulting in the glycosylated isoform CRTAC1-A which represents a new extracellular matrix molecule of articular cartilage (PMID: 17074475). CRTAC1 was downregulated in bladder cancer tissues and cells (PMID: 34818994).

实验方案

Product Specific Protocols
WB protocol for CRTAC1 antibody 30251-1-APDownload protocol
IHC protocol for CRTAC1 antibody 30251-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols