验证数据展示
经过测试的应用
| Positive WB detected in | Neuro-2a cells, HeLa cells, HEK-293T cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
27647-1-AP targets CRTC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Immunogen | CRTC1 fusion protein Ag26494 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | CREB regulated transcription coactivator 1 |
| Synonyms | TORC1/CRTC1, TORC-1, TORC1, TORC 1, MECT1 |
| Calculated Molecular Weight | 67 kDa |
| Observed Molecular Weight | 68-75 kDa |
| GenBank Accession Number | BC017075 |
| Gene Symbol | CRTC1 |
| Gene ID (NCBI) | 23373 |
| RRID | AB_2880938 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6UUV9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CRTC1, also named as Transducer of CREB protein 1, is a 634 amino acid protein, which belongs to the TORC family. CRTC1 is highly expressed in adult and fetal brain and is low expressed in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney. CRTC1 localizes in Cytoplasm when is phosphorylated by SIK or AMPK and when sequestered by 14-3-3 proteins (PubMed:16817901). it translocates to the nucleus on Ser-151 dephosphorylation, instigated by a number of factors including calcium ion and cAMP levels. CRTC1 as a Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. The calculated molecular weight of CRTC1 is 68 kDa, but the modified protein is about 70-75 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CRTC1 antibody 27647-1-AP | Download protocol |
| IHC protocol for CRTC1 antibody 27647-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy Keratinocyte autophagy enables the activation of keratinocytes and fibroblasts and facilitates wound healing. | ||
Brain Res The α1 adrenoceptor antagonist prazosin potentiates morphine induced conditioned place preference in rats |
