验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, mouse liver tissue, HEK-293 cells, mouse kidney, rat kidney |
| Positive IHC detected in | human skin cancer tissue, human gliomas tissue, rat kidney tissue, human urothelial carcinoma tissue, mouse lung tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12497-1-AP targets TORC2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, bovine |
| 免疫原 | TORC2 fusion protein Ag3167 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CREB regulated transcription coactivator 2 |
| 别名 | CRTC2,TORC2, CRTC2, Transducer of regulated cAMP response element-binding protein 2, Transducer of CREB protein 2, TORC 2 |
| 计算分子量 | 693 aa, 73 kDa |
| 观测分子量 | 73 kDa |
| GenBank蛋白编号 | BC053562 |
| 基因名称 | TORC2 |
| Gene ID (NCBI) | 200186 |
| RRID | AB_2260910 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q53ET0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CRTC2, also named as TORC2, belongs to the TORC family. It is a transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. It acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. CRTC2 enhances the interaction of CREB1 with TAF4. Ir regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. CRTC2 regulates the expression of specific genes such as the steroidogenic gene, StAR. TORC2 was recently shown to be an important regulator of gluconeogenesis in the livers of mammals. It is one of the other key regulators of CRE-dependent MIE gene expression in NT2 cells. This regulation is linked to VIP-induced TORC2 dephosphorylation and translocation to the nucleus. (PMID: 19369332, 20504934 ) .
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TORC2 antibody 12497-1-AP | Download protocol |
| IHC protocol for TORC2 antibody 12497-1-AP | Download protocol |
| IF protocol for TORC2 antibody 12497-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) cAMP-Induced Nuclear Condensation of CRTC2 Promotes Transcription Elongation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease.
| ||
Mol Cell LKB1 controls inflammatory potential through CRTC2-dependent histone acetylation | ||
Am J Transplant The tacrolimus-induced glucose homeostasis imbalance in terms of the liver: From bench to bedside. | ||
J Neurosci Clock and light regulation of the CREB coactivator CRTC1 in the suprachiasmatic circadian clock. | ||
Biochem Pharmacol Piperlongumine, a natural alkaloid from Piper longum L. ameliorates metabolic-associated fatty liver disease by antagonizing the thromboxane A2 receptor | ||
